The codon chart outlines the various codon combinations and their corresponding amino acids. To use the codon chart, for example, if the first codon position contains uracil (U), the second contains adenine (A), and the third contains cytosine (C), the resulting codon, UAC, represents the a...
through comparisons of protein amino acid sequences, functional domains and structures predicted by AlphaFold44, we found that RH11, RH37, and RH52 are orthologous to the yeast Ded1p and human DDX3X (Extended Data Fig.8b–d). The sequence and structural homology to the yeast Ded1p also ali...
Target/Organ: ADRB2, adrenoceptor beta 2; AGXT, alanine-glyoxylate and serine-pyruvate aminotransferase; ALAS1, 5′-aminolevulinate synthase 1; APOB, apolipoprotein B; BCL2L12, BCL2 like 12; C5, complement C5; CBLB, Cbl proto-oncogene B; CTGF, connective tissue growth factor; EPHA2, ...
3 × Flag tag amino acids (DYKDDDDK) were also fused at the end of RBM47 to construct the RBM47Flag fusion protein. All plasmid vectors were transfected with Lipofectamine 2000 (Thermo Fisher) following the protocol of the manufacturer. RNA and DNA extraction The extraction of the total ...
By comparing amino acid sequences of the METTL3 MTase domain across different species,17 we located highly conserved residues within amino acids 380–500 of METTL3 (Figure 3D). We hypothesized that certain specific residues within the MTase domain may be vital for METTL3 function. To test this...
Nucleotide and deduced amino acid sequence of Cimex lectularius P450 (Cl CYP397A1V2) (A). The first line represents nucleotide sequence and the second line represents amino acid sequence. The amino acids highlighted in grey indicate start codon, signature motifs (helix I;[A/G]GX[E/D]T[T...
npr1-1 harbors H334Y7; npr1-0 (SALK_204100) is a null mutant that has a T-DNA insertion in the first exon of the NPR1 gene16; and npr1-6 (SAIL_708_F09) contains a T-DNA insertion in the third exons of the NPR1 gene, which allows the expression of amino acids 1-466 of NPR1...
2C). UPR program was characterized by key genes regulating protein folding and endoplasmic reticulum (ER) stress, and metabolic-associated program was featured by high expression of metabolic genes involved in glycolysis and amino acids metabolism (Fig. 2C and Additional file 1: Table S2). ...
As discussed above, the non-coding transcripts that do not encode proteins and are more than 200 nucleotides in length are known as long non-coding RNAs (lncRNAs). The length of a lncRNA can be more than 2 Kb while their coding potential is less than 100 amino acids [5]. Kaur and co...
As used herein “polar metabolites” refers to all hydrophilic metabolites showing a capacity to interact with polar solvents, in particular with water or with other polar groups, such as sugars, amino acids, organics acid, etc. As used herein “non-polar metabolites” refers to all hydrophobic...