Pie chart shows the fraction of hyper CGIs targeted by PRC2 in ESCs. White dots denote the median, edges the interquartile range (IQR) and whiskers either 1.5× IQR or minima/maxima (if no point exceeded 1.5× IQR; minima/maxima are indicated by the violin plot range). c, Scatter ...
To narrow down the KIND2-binding site, we substituted each amino acid in the TLN1-F3 α1-helix individually with alanine or glutamic acid to identify THD1 mutants that affect affinities for β3-CT in the presence of near-saturation binding concentrations of KIND2. MST measurements revealed tha...
This chart represents amino acids that are coded from different combinations of mRNA codons. Second Base First Base Third Base UUU UUC UUA UUG CUU CUC CUA CUG AUU AUC AUA AUG GUU GUC GUA GUG Phenylalanine Phenylalanine Leucine Leucine Leucine Leucine Leucine Leucine Isoleucine Isoleucine Isoleucine...
Multiple protein isoforms, containing distinct amino acid (AA) sequences, can arise from the same gene through mechanisms such as alternative promoter usage or splicing [2] and can exhibit different stabilities, molecular binding capabilities, and functional effects [3, 4]. Many protein isoforms ...
Substitution of valine with more hydrophobic amino acid leucine at position 1230 of the transmembrane domain, having role in S protein binding to the viral envelope, could strengthen the interaction of S protein with the viral envelope and also increase the deposition of S protein to the viral ...
Skp2-mediated RagA ubiquitination elicits a negative feedback to prevent amino-acid-dependent mTORC1 hyperactivation by recruiting GATOR1. Mol. Cell 58, 989–1000 (2015). Article CAS PubMed PubMed Central Google Scholar Wang, J. Y. et al. Skp2-mediated stabilization of MTH1 promotes ...
The CDKs range in size from approximately 250 amino acid residues, just encompassing the catalytic serine/threonine kinase domain, to proteins of more than 1,500 residues, with amino- and/or carboxy-terminal extensions of variable lengths (Figure2). Like all kinases, CDKs have a two-lobed st...
by single counting of all GO-terms assigned to clusters in the cadmium (red) and phenanthrene (blue) suppressive subtractive hybridization (SSH) library, counted by CateGOrizer [27], Each of the eight corners of the radar plots represent an ancestor term (plots designed with Chart Tool [69]...
The samples were then washed twice, resuspended in FACS buffer (PBS, 2% FCS and 2 mM ethylenediaminetetraacetic acid) and loaded for flow sorting. The gating strategy was as follows: main population > single cells > living cells (7-aminoactinomycin D/DAPI negative) > FVFneg,...
. Cells were treated for 24 hours with doxycycline (dox) to induce expression of mRuby2-3×FLAG-tagged CENP-N (WT or mutant) and/or with indole acetic acid (IAA) to deplete endogenous auxin-inducible degron (AID)-tagged CENP-N-GFP as indicated.e,f, Normalized centromeric fluorescence ...