RISC loading complex(RLC):是一种促使RISC形成的复合物。 RLC有方向性地调节小RNA双螺旋,为以后的RISC组装作好铺垫。siRISC loading complexes (siRLCs)在果蝇中研究最多。 有研究者认为在果蝇中的siRLCs包含DCR2-R2D2异型二聚体和siRNA双螺旋;R2D2部分是非对称性的感受器,为RISC组装调整好siRNA的方向。 miRISC...
产品名称:RISC-loading complex subunit TARBP2 ELISA Kit 检测范围:0.312-20 ng/mL. 保存条件:2-8℃低温保存 保质期:6个月,所有试剂盒均提供批次。 试剂盒成分:酶标板,试剂,标准品等。 选型: 产品规格:96T/48T 96T指可以做94个标本-2个标准对照-84个样本 ...
Berkeley Researchers Get First Look At Gene-Silencing Human RISC-Loading ComplexLynn Yarris
In humans, Argonaute2 (AGO2) assembles with the guide RNA–generating enzyme Dicer and the RNA-binding protein TRBP to form a RISC-loading complex (RLC), which is necessary for efficient transfer of nascent siRNAs and miRNAs from Dicer to AGO2. Here, using single-particle EM analysis, we...
英文名称: RISC-loading complex subunit TARBP2 ELISA Kit 英文同义词: RISC-loading complex subunit TARBP2 ELISA Kit CAS号: 分子式: 分子量: 0 EINECS号: Mol文件: Mol File FOR RISC-LOADING COMPLEX SUBUNIT TARBP2 ELISA KIT 信息错误报告 提交 按...
(2′-OMe) MePS2 on one nucleotide that significantly enhances potency and resistance to degradation for various siRNAs. We find enhanced potency stems from an unforeseen increase in siRNA loading to the RNA-induced silencing complex, likely due to the unique interaction mediated by 2′-OMe and ...
Alternatively, exogenous siRNA enters the pathway at this point, where it is recognized by the TRBP/PACT/Ago2 and Dicer complex, although its loading into RISC is not as efficient as that of hairpin pre-miRNAs or 27-mer Dicer substrate RNA duplexes (Elbashir et al., 2001, Gregory et al...
The present invention provides double stranded compositions wherein one of the strands is useful in, for example, influencing the preferential loading the opposite strand into the RISC (or cleavage) complex. In particular, the present invention provides oligomeric compounds that comprise chemical ...
2′-OMe-phosphorodithioate-modified siRNAs show increased loading into the RISC complex and enhanced anti-tumour activity. Nature communications. 2014; 5 :3459. doi: 10.1038/ncomms4459. [ Cross Ref ]’’-OMe-phosphorodithioate-modified siRNAs show increased loading into the RISC complex and ...
MicroRNAs (miRNAs) regulate expression of their target mRNAs through the RNA-induced silencing complex (RISC), which contains an Argonaute (Ago) family protein as a core component. In Drosophila melanogaster, miRNAs are generally sorted into Ago1-containing RISC (Ago1-RISC). We established a ...