Targeted gene silencing by RNA interference (RNAi) requires loading of a short guide RNA (small interfering RNA (siRNA) or microRNA (miRNA)) onto an Argonaute protein to form the functional center of an RNA-induced silencing complex (RISC). In humans, Argonaute2 (AGO2) assembles with the ...
(Electron microscopy structure of the human RISC-loading complex, with the L-shaped Dicer enzyme shown as a wire map and the Argonaute2 protein, shown in purple. ) 人类细胞中的小分子RNA通常由20到2000个核苷酸组成,小分子RNA包括:小干扰RNAs(small interfering RNAs,siRNA)和microRNAs(miRNA)。这些siR...
解析 RISC由Dicer酶,Argonaute蛋白,siRNA等多种生物大分子装配而成。RISC 的组装是 RNAi 和 miRNA 途径的中心环节,包括 small RNA 的形成,small RNA 进入RISC 装载复合体( RISC loading complex, RLC ) 并进而转变为有沉默目标 mRNA 活性的 RISC [1] 。
(2′-OMe) MePS2 on one nucleotide that significantly enhances potency and resistance to degradation for various siRNAs. We find enhanced potency stems from an unforeseen increase in siRNA loading to the RNA-induced silencing complex, likely due to the unique interaction mediated by 2′-OMe and ...
装载后的 siRNA 解链,正义链被降解,pre-RISC 活化为成熟的 RISC,由反义链引导其靶向降解目的 RNA。本手稿对近年来 RISC 的组装,活化过程及其重要组分的结构功能的研究进展进行综述。关键词 RNA 诱导基因沉默复合物, siRNA, RNA 干扰,Ago 蛋白Study of siRNA-induced Silencing ComplexCui Shiquan Hou Gan*Huang...
参考答案: RISC由Dicer酶,Argonaute蛋白,siRNA等多种生物大分子装配而成。RISC 的组装是 RNAi 和 miRNA 途径的中心环节,包括 small RNA 的形成,small RNA 进入RISC 装载复合体( RISC loading complex, RLC ) 并进而转变为有沉默目标 mRNA 活性的 RISC [1] 。 复制 纠错...
内容提示: 小干扰 RNA ( smallinterferingRNA , siRNA )和微RNA ( microRNA , miRNA ) 是 RNA 干扰 ( RNAinterference,RNAi ) 途径中的 2 种重要的小 RNA( smallRNA )。在发挥转录后基因沉默效应之前,siRNA 和 miRNA 需进入相应的 RNA 诱导沉默复合体 ( RNA-inducedsilencingcomplex,RISC ) siRISC 和mi...
小干扰RNA(smallinterferingRNA,siRNA)和微中心环节,包括smallRNA的形成,smallRNA进入 RNA(microRNA,miRNA)是RNA干扰(RNARISC装载复合体(RISCloadingcomplex,RLC)并进而 转变为有沉默目标mRNA活性的RISC。本文将就近 年来有关RISC的组装及其功能的研究作一综述。
RNA interference (RNAi), the sequence-specific silencing of gene expression, is directed by small RNAs acting in concert with several proteins in the RNA-induced silencing complex (RISC). Although human RISC activity can be reconstituted in vitro using single-stranded siRNA and recombinant Argonaute...
RISC RNA-induced silencing complex RNA 诱导的沉默复合体 PKR Protein kinase R 蛋白激酶R dsRNA Double strand RNA 双链RNA siRNA Small interference RNA 小干扰RNA miRNA microRNA 微小RNA Ago2 Argonaute family protein 2 Argonaute 家族蛋白2 dsRBD Double strand RNA binding domain 双链RNA 结合结构域 ...