BCA法和Commassie法各有优势,在不知道蛋白样本缓冲液成分的情况下可以两种方法配合使用,以消除定量的误差。 (Radio Immunoprecipitation Assay)RIPA裂解液(RIPA Lysis Buffer)是一种传统的细胞组织快速裂解液。RIPA裂解液裂解得到的蛋白样品可以用于常规的Western、IP等。 RIPA使用方法 对于培养细胞样品: 1. 融解RIPA裂解...
3 Customer Q&As for RIPA Lysis Buffer Question In the AR0105-100 protocol it mentions "transferring the supernatant to a new tube for further analysis." What the supernantant can be analyzed for and what type of anaylsis would be useful?" ...
Protocol Note: RIPA buffer (Part No. RIPA-50) is provided as a 10X solution. Dilute to 1X with dH20 prior to your experiment. A.Cell Culture: It is recommended that cells are cultured to 80-90% confluency prior to performing cell lysis. Cells should be washed free of serum proteins ...