II. Composition/Information on Ingredients: Other than the ingredients listed below, this product is a mixture that contains less than 1% hazardous chemicals. According to 29CFR 1910.1200(d), ingredients at less than 1% concen- tration are not considered to be hazardous. ...
Lysis:For lysis of adherent cells, we recommend the following: (all reagents and lysates must be kept cold).Treat cells as desired.Wash plate with PBS to remove residual media.Add 400 µl of 1x RIPA buffer/10 cm dish.Incubate plate on ice for 5 minutes.Scrape cells.Sonicate briefly.Ce...