BCA法和Commassie法各有优势,在不知道蛋白样本缓冲液成分的情况下可以两种方法配合使用,以消除定量的误差。 (Radio Immunoprecipitation Assay)RIPA裂解液(RIPA Lysis Buffer)是一种传统的细胞组织快速裂解液。RIPA裂解液裂解得到的蛋白样品可以用于常规的Western、IP等。 RIPA使用方法 对于培养细胞样品: 1. 融解RIPA裂解...
ripa buffer 读音:美英 ripa buffer基本解释 细胞裂解液 分词解释 ripa[医] 缘线(室管膜的翻转处),丘脑带 buffer缓冲器
0 Reviews For RIPA Lysis Buffer Customer Q&As Have a question? Find answers in Q&As, reviews. Can't find your answer? Submit your question 3 Customer Q&As for RIPA Lysis Buffer Question In the AR0105-100 protocol it mentions "transferring the supernatant to a new tube for further analysis...
MilliporeTechnicalPublications Protocol:RIPABuffer:PreparationofModifiedRadioimmunoprecipitation(RIPA)BufferCatalogueNumber: mcproto402 Year:..
裂解30min后4度冷冻高速离心20min,弃沉淀,上清按比例加入5Xloading buffer沸水煮15min,即可上样,或-20度保存,用的时候沸水煮5min就行... 实验材料 肿瘤细胞试剂、试剂盒 PBS生理盐水裂解液蛋白酶抑制剂仪器、耗材 培养瓶细胞刮棒离心管低温离心机-80℃冰箱探针型超声波恒温水浴锅实验步骤 一、 裂解细胞1. 方向...
Protocol Note: RIPA buffer (Part No. RIPA-50) is provided as a 10X solution. Dilute to 1X with dH20 prior to your experiment. A.Cell Culture: It is recommended that cells are cultured to 80-90% confluency prior to performing cell lysis. Cells should be washed free of serum proteins ...
8.根据各样品浓度计算1×loading buffer体积,将各样品浓度调为一致;并计算5×loading buffer的体积,加入各管中。混匀后沸水煮15min,冰上分装,存于-20℃。 Tips 1.前面提到收集蛋白于进口Axygen EP管中,是为了防止煮沸蛋白时EP管盖子弹开,液体溅出而改变蛋白浓度。
建议:蛋白样品在加入SDS之前最好统一稀释至浓度为2.5µg/uL(因为最终上样体积为10uL,上样蛋白为20ug,其中在变性前,1次实验的蛋白样本为8µL,需要加入含有5XSDS的Loading Buffer为2uL,因此蛋白浓度最终就是2µg/µL)。每次制备的样品需要按3次实验进行分装,即每个试管中装24uL,每支的浓度为2.5µg/...