Human RHPN1-AS1 qPCR Primer Pair,即人RHPN1-AS1 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time ...
A tumor xenograft model in nude mice was established to confirm the function of RHPN1-AS1 in vivo. RHPN1-AS1 was significantly upregulated in a number of UM cell lines compared with the normal human retinal pigment epithelium (RPE) cell li...
说明RHPN1-AS1通过靶向MiR-299-3p/TNFSF12通路调控吉非替尼耐药株的耐药机制.结论:在这项研究中,我们首先采用实时定量PCR对44例吉非替尼敏感和40例吉非替尼耐药的患者肿瘤组织标本进行了组织中表达水平的检测,结果显示在吉非替尼耐药组中RHPN1-AS1的表达明显低于吉非替尼敏感组.为了进一步确认,我们又在PC9细胞...
western blot assays. Knockdown of RHPN1-AS1 delivered by lentivirus system inhibited MCF-7 cell proliferation indicated by the cell proliferation and colony formation assays, and the knockdown of RHPN1-AS1 enhanced P53 protein expression in MCF-7 and MDA-MB-231 cells. In addition, luciferase repo...
RHPN1-AS1gefitinibNSCLCAlthough epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) gefitinib has exhibited notable clinical efficacy in non-small cell lung cancer (NSCLC) patients. However, its therapeutic efficacy is ultimately limited by the development of gefitinib resistance. The...
lncRNA RHPN1-AS1miR-485-5pProliferationMetastasisHepatocellular carcinomaIt is reported that long noncoding RNA RHPN1-AS1 (lncRNA RHPN1-AS1) functions as an oncogene among multiple types of cancers; however, the effect of lncRNA RHPN1-AS1 in hepatocellular carcinoma (HCC) is left to be ...
qRT-PCR assay was used to examine RHPN1-AS1 expression in CRC cell lines and non-tumor cell line. The transfection efficiency of sh-RHPN1-AS1#1/#2 plasmid was detected by qRT-PCR. , CCK-8 and EdU were performed to investigate cell proliferation. Flow cytometry was used to determine ...
侵袭凋亡目的:探讨lncRNA RHPN1反义RNA1(RHPN1-AS1)靶向miR-485-5p对骨肉瘤细胞增殖,凋亡,迁移,侵袭的影响和机制.方法:实时荧光定量PCR(qRT-PCR)检测20例骨肉瘤组织与其对应的癌旁组织,人正常成骨细胞hFOB1.19以及3种骨肉瘤细胞(U-2OS,SAOS-2,HOS)中RHPN1-AS1和miR-485-5p的表达水平.利用脂质体转染法将...
The long noncoding RNA, RHPN1 antisense RNA 1 (RHPN1-AS1), performs important regulatory actions in the progression of many human cancers. In this study, we aimed to analyze RHPN1-AS1 expression in osteosarcoma (OS) and to assess the influence of RHPN1-AS1 knockdown on the malignant ...
RHPN1‐AS1miR‐345‐5pprognosispathwayLncRNAs have been reported to be involved in the initiation and progression of cholangiocarcinoma (CCA), although the mechanisms by which this occurs remains unknown. The current study aimed to reveal the clinico﹑athological relationship of RHPN1〢S1 expressed ...