【Science:新研究揭示Ctf18-RFC装载PCNA用于前导链DNA合成的机制】DNA聚合酶(Pol),如前导链Pol(Polε)和滞后链Pol(Polδ),在全酶背景下与增殖细胞核抗原(PCNA)DNA滑动夹一起发挥活性和加工能力。PCNA夹是一个封闭的环,需要通过使用ATP的夹装载因子在DNA周围打开和关闭。一般认为,前导链和滞后链 DNA 上的 PCN...
增殖细胞核抗原(PCNA)是一种在DNA复制过程中至关重要的蛋白质,它通过包裹DNA,将DNA聚合酶(Pols)固定在DNA上,以提高其合成效率。在DNA复制中,Ctf18-RFC(复制因子C变体)作为PCNA加载器,具有独特的特性,专门为主链DNA聚合酶(Polε)服务。尽管PCNA加载机制已经被广泛研究,但Ctf18-RFC如何特异性地识别并加载PCNA到Pol...
The eukaryotic processivity clamp, proliferating cell nuclear antigen (PCNA), exists mostly as a closed ring in solution. Replication factor C (RFC), a five-subunit ATP-dependent protein complex, mediates PCNA opening in solution (assembly stage) and closing onto the primer-template (disassembly ...
Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct
M. (2012) ATP binding and hy- drolysis-driven rate-determining events in the RFC-catalyzed PCNA clamp loading reaction. J. Mol. Biol. 416, 176 -191Sakato, M., Zhou, Y. & Hingorani, M. M. (2012). ATP binding and hydrolysis-driven rate-determining events in the RFC-catalyzed PCNA ...
Replication factor C (RFC) is an AAA + heteropentamer that couples the energy of ATP binding and hydrolysis to the loading of the DNA polymerase processivity clamp, proliferating cell nuclear antigen (PCNA), onto DNA. RFC consists of fiv... Johnson,Aaron,Yao,... - 《Journal of Biological...
However, its persistent localization at stalled NER complexes suggests that RFC remains targeted to the repair complex even after loading of PCNA. We speculate that RFC associates with the downstream 5' phosphate after loading; such interaction would prevent possible signaling events initiated by the ...
在原核DNA复制中,可与单股DNA链结合的是( ) A、PCNA B、SSB C、DnaA D、DnaB E、RFC 点击查看答案&解析 手机看题 你可能感兴趣的试题 单项选择题 Where is your brother?He () to the dining room. A.had goneB.had beenC.has beenD.has gone 点击查看答案&解析 手机看题 单项选择题 患者,...
该研究提出的模型强调了Ctf18-RFC和Polε在前导链DNA复制过程中的协同作用。Ctf18-RFC通过其特异的钩状结构与Polε结合,并利用Polε的稳定作用来加速PCNA的装载。这一过程不仅解释了为什么真核细胞需要专门的Ctf18-RFC来装载前导链上的PCNA,也揭示了DNA复制机器在进化过程中如何通过精细的分子机制来优化其效率和准...
The DNA sliding clamp PCNA is a multipurpose platform for DNA polymerases and many other proteins involved in DNA metabolism. The topologically closed PCNA ring needs to be cracked open and loaded onto DNA by a clamp loader, e.g., the well-studied pentameric ATPase complex RFC (RFC1-5). ...