增殖细胞核抗原(PCNA)是一种在DNA复制过程中至关重要的蛋白质,它通过包裹DNA,将DNA聚合酶(Pols)固定在DNA上,以提高其合成效率。在DNA复制中,Ctf18-RFC(复制因子C变体)作为PCNA加载器,具有独特的特性,专门为主链DNA聚合酶(Polε)服务。尽管PCNA加载机制已经被广泛研究,但Ctf18-RFC如何特异性地识别并加载PCNA到Pol...
【Science:新研究揭示Ctf18-RFC装载PCNA用于前导链DNA合成的机制】DNA聚合酶(Pol),如前导链Pol(Polε)和滞后链Pol(Polδ),在全酶背景下与增殖细胞核抗原(PCNA)DNA滑动夹一起发挥活性和加工能力。PCNA夹是一个封闭的环,需要通过使用ATP的夹装载因子在DNA周围打开和关闭。一般认为,前导链和滞后链 DNA 上的 PCN...
DNA聚合酶delta和DNA聚合酶epsilon对DNA模板的延伸需要辅助蛋白增殖细胞核抗原(PCNA)和复制因子C(RFC)rfc,又称激活因子1,是一种由140、40、38、37和36kd五个不同亚基组成的蛋白质复合物。这个基因编码37kd亚单位。该亚单位与36和40kDa亚单位形成核心复合物。核心复合物具有DNA依赖的atp酶活性,PCNA在体外系统中可...
The eukaryotic processivity clamp, proliferating cell nuclear antigen (PCNA), exists mostly as a closed ring in solution. Replication factor C (RFC), a five-subunit ATP-dependent protein complex, mediates PCNA opening in solution (assembly stage) and closing onto the primer-template (disassembly ...
Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, ...
p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer...
This subunit can interact with the C-terminal region of PCNA and form a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. 实验方案 Product Specific Protocols IP protocol ...
Replication factor C subunit 5 (RFC5), also known as activator 1, can recruit proliferating cell nuclear antigen(PCNA) onto the DNA polymerase S-phase checkpoint complex. In eukaryotes, RFC5 is involved in repairing mismatches, DNA double helix damage, nucleotide excision, and regulating the cell...