TaKaRaCode:D2639A ReverseTranscriptaseM-MLV (RNaseH¯)说明书宝生物工程(大连)有限公司目录内容页码●制品说明 1 ●包装 1 ●保存 1 ●酶贮存溶液 1 ●起源 1 ●活性定义 1 ●纯度 1 ●用途 1 ●添附Buffer组成 1 ●1st-StrandcDNA合成的实验操作方法 2 ●使用λRNA进行RT-PCR反应的实验例 2 ●使用...
Reverse Transcriptase M-MLV
PrimeScript Reverse Transcriptase是Takara公司依据M-MLV (Moloney Murine Leukemia Virus)来源的RTase开发的一种反转录酶。本酶具有很强的延伸能力,能够有效地合成1st-strand cDNA,即使对有复杂二级结构的RNA,在42℃条件下也能得到良好的反应效果,无需进行高温反转录反应,高温的反转录反应会导致RNA的降解。本酶适合于...
Reverse transcriptase (RTase) is a group of DNA polymerases that catalyze the synthesis of complementary DNAs from RNA templates efficiently, thus performing reverse transcription. Reverse transcriptase was first found in purified virions of murine leukemia virus (MLV) by Baltimore (1) and in Rouse...
Figure 1. High yields of cDNA over a broad temperature range.cDNA synthesis was performed using(A)Maxima H Minus Reverse Transcriptase (20U)(B)Takara PrimeScript™ Reverse Transcriptase,(C)Promega GoScript™ Reverse Transcriptase, and(D)NEB ProtoScript™ II Revers...
200 U / ml M-MLV Reverse transcriptase (GIBCO Part No.28025-021) 500 mg / ml oligo (dT) 15 primer (Promega Cat. No. C110A 9362612) 10 mM dNTP mix RNase-free DNase I (TaKaRa Code No. 2215 CA) 5 U / ml Ex Taq polymerase and 10 × buffer (TaKaRa Code No. RR001D CA) ...
than the wild type M-MLV reverse transcriptase having the amino acid sequence represented by SEQ. ID. NO: 1. 5. A gene encoding the reverse transcriptase having improved thermostability of claim 1. 6. The gene encoding the reverse transcriptase according to claim 5, wherein the gene is ...
For mRNA expression analysis, cDNAs were synthesized from 1 μg of total RNA using the AMV reverse transcriptase (TaKaRa) and oligo dT primers (TaKaRa). The reverse transcription reaction was carried out at 16 °C for 5 min, 42 °C for 60 min and 85 °C for 5 min. ...
SMART MMLV Reverse Transcriptase is an ultra-pure Moloney Murine Leukemia Virus reverse transcriptase designed to virtually eliminate contaminating RNases and other nucleases that are often introduced into the RT reaction with the enzyme. SMART MMLV RT helps prevent degradation of template RNA, while ...
The prime editor system composed of Streptococcus pyogenes Cas9 nickase (nSpCas9) and engineered Moloney murine leukaemia virus reverse transcriptase (M-MLV RT) collaborates with a prime editing guide RNA (pegRNA) to facilitate a wide variety o