Direct coupling of a nano-high-performance liquid chromatography column to an ion trap designed for a gas chromatography/mass spectrometry system . Moneti G, Pieraccini G, Bartolucci G, Guida G, Seraglia R, Traldi P . Rapid Commun Mass Spectrom ( 2001 ) 15 1609 ....
DNA synthesisoccurs duringchromosome replicationand duringDNA repair; replication of the genome occurs during the S phase of the cell cycle if cyclin-Cdk complexes are present to allow the cell to pass the G1checkpoint, and repair occurs throughout the cell cycle wherever damage is detected. ...
The recombinant proteins were purified from the supernatant using HisTrap HP column (GE Healthcare), followed by an ion exchange chromatography column (GE Healthcare) on an AKTA Purifier 10 system (GE Healthcare) according to the manufacturer’s protocol, and then concentrated to about 20 mg...
deliveredtoreversed-phasehighperformance liquidchromatography(HPLC) and ion-trap mass spectrometry [...] chrom-china.com chrom-china.com 利用胶内胰蛋白酶消化技术将SDS-PAGE胶内分离的蛋白质降解为相应的肽段,然后以反相高效液相色谱分离和离子阱质谱仪鉴定肽段。
The maturation of single-cell transcriptomic technologies has facilitated the generation of comprehensive cellular atlases from whole embryos1–4. A majority of these data, however, has been collected from wild-type embryos without an appreciation for th
The maturation of single-cell transcriptomic technologies has facilitated the generation of comprehensive cellular atlases from whole embryos1–4. A majority of these data, however, has been collected from wild-type embryos without an appreciation for th
For each analysis, 500 ng of each sample was loaded into a trap column Acclaim PepMap 2 cm * 100 μm i.d., C18, 3 μm, 100 A (Thermo Fisher Scientific) at 5 μl /min with aqueous solution containing 0.05% (v/v) trifluoroacetic acid and 1% acetonitrile. After 3 min, the trap ...
purifying rT3 in said supernatant by subjecting said supernatant to a reverse-phase high performance liquid chromatography (RP-HPLC) column; wherein said purifying comprises introducing an aqueous solution to said column immediately prior to introducing said supernatant; iii. eluting rT3 from said RP-...
Rivier, J . 1978 . Use of trialkyl ammonium phosphate (TRAP) buffers in reverse phase HPLC for high resolution and high recovery of peptides and proteins . J. Liq. Chromatogr. 1 :343-366.Rivier J. Use of trialkyl ammonium phosphate (TAAP) buf- fers in reverse phase HPLC for high ...
In addition, a Cation H Cartridge 125-0129 column was used to provide guard column. The column temperature and the detector temperature were respectively set as 60°C and 50°C. A HPX-87H column was used with 5 mM H2SO4 as the mobile phase (20 μL injection, 0.6 mL/min flow rate,...