The adeno-associated virus (AAV) vector has become an increasingly popular platform for gene delivery to neural cells in vivo, and it is therefore meaningful to develop a new type of retrograde gene transfer approach based on a tropism-free AAV vector system. New Method The wild-type or ...
Retrograde axonal transport property of adeno-associated virus and its possible application in future Microbes and Infection Journal2021, Microbes and Infection Jingjing Wang, Liqin Zhang 1 Application of retrograde ability of AAV Articles published on Pubmed or Web of Science describing the application ...
Adeno-associated virus (AAV) vectors often undergo long-distance axonal transport after brain injection. This leads to transduction of brain regions distal to the injection site, although the extent of axonal transport and distal transduction varies widely among AAV serotypes. The mechanisms driving th...
compartment using a retrograde eGFP rabies virus and quantified spine density before and 24 h following axotomy and compared these measurements to cultures with vehicle applied to axons during axotomy (Fig.6a, b). Strikingly, we found that local activity blockade at the injury site prevented ax...
To enable retrograde gene transfer, pseudotyping of lentiviral vectors with rabies virus glycoprotein (RV-G) was attempted12,13. Later, fusion envelope glycoproteins (FuG) consisting of distinct combinations of RV-G and VSV-G were developed to enhance the efficiency of retrograde transgene delivery...
(GAD2). AAV2/9-DIO-mCh-gK was applied as the helper virus to assist the Cre-dependent anterograde monosynaptic tracing of H129-dgK-G4 from a specific neuron type. Controlled by the double floxed inverted orientation (DIO) Cre-On system, AAV2/9-DIO-mCh-gK expresses mCherry and gK ...
A similar neuroprotective effect was observed in transgenic ALS mice following intramuscular injection of an adeno-associated virus (AAV) carrying the gene for GDNF (Wang et al.2002). Wang et al. (2002) noticed that the addition of AAV-GDNF led to longer expression of transgenic GDNF in myofi...
However, because of its attractive safety profile and durable gene expression, adeno-associated virus (AAV) remains the only vector to have been applied to the human nervous system for the treatment of neurodegenerative disease. Nonetheless, only a very small fraction of intramuscularly injected AAV...
Retrograde delivery of adeno-associated virus (AAV) expressing GFP (AAV-GFP) to phrenic motoneurons by intrapleural injection.Heather M. GranseeWenZhi ZhanGary C. SieckCarlos B. Mantilla
The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We ...