【维真生物-AAV病..病毒产品:AAV(retro)-hSyn-GFP;注射方法:局部注射小鼠脑室某区;病毒滴度:4.77×10E13VG/mL;注射量:100nL;取材时间:注射后2周取材拍摄;结果:病毒被轴突吸收,逆行到
【AAV病毒应用】AAV(retro)感染小鼠海马区 病毒产品:维真生物-AAV(retro)-hSyn-GFP; 注射方法:局部注射小鼠脑室某区; 病毒滴度:4.77×10E13VG/mL; 注射量:100nL; 取材时间:注射后2周取材拍摄; 结果:病毒被轴突吸收,逆行到胞体,到达海马CA1区,得到了十分满意的实验结果 【维真生物-AAV病毒应用】注射位点 04:0...
Secondly, AdLacZ (2脳1012vp) and AAV2-GFP (2脳1013vg) gene transfers were performed to study gene transduction efficacy of the method. By retrograde injections with simultaneous coronary arterial occlusions, both adenovirus (Ad) and adeno-associated virus (AAV) vectors were shown to deliver an...
Conversely, it has been shown that a 3 × flag tag, when fused to the C-terminus of the ORF2p, is able to recover ORF2 stability and function that is lost with the single GFP fusion (Dai et al., 2014). In our experiments, the ORF2p fusion constructs retained the ability to ...
89 VSV-p1-GFP was identified in a screen for selective cytotoxicity in glioma cells and not normal glial cells, even in the presence of IFN-α, and found to be nonneurovirulent in mice.89 Recently, a semireplication-competent VSV vector system was created (srVSV; Table 19.1), where ...
Taking advantage of this phenotype, CD4+ T cells were co-infected with two HIV-1 vectors (WT-GFP and N74D-mCherry), which were identical except for the CA N74D mutation. Compound libraries were screened to find molecules that selectively inhibited infection of WT over N74D, which, by ...
病毒产品:维真生物-AAV(retro)-hSyn-GFP; 注射方法:局部注射小鼠脑室某区; 病毒滴度:4.77×10E13VG/mL; 注射量:100nL; 取材时间:注射后2周取材拍摄; 结果:病毒被轴突吸收,逆行到胞体,到达海马CA1区,得到了十分满意的实验结果 【维真生物-AAV病毒应用】注射位点 ...
Anterograde tracing of RTNNmb neurons was complemented using retrograde transport of Cre-recombinase (AAVrg-Cre) in R26-loxSTOPlox-L10-GFP mice (eGFP-reporter) mice combined with labelling of transcript for Nmb by FISH. Small volumes (3-6 nL) of AAVrg-Cre were unilaterally injected in the...