The formula for doubling time calculation in the 24 h was 24*Ln(2)/Ln(T2/T1), where T1 and T2 represent the cell growth values corresponding to 12 and 36 h, respectively. PAS staining Four-micrometer thick FFPE sections were processed for Periodic Acid Schiff (PAS) staining. Briefly, ...
It should also be noted that including a UF in the AET calculation does nothing to resolve RF Problem 2: Quantitative error. It is therefore strongly desirable to mitigate response factor variation and with it the need for large UF values. This would provide enhanced reliability for the ...
Abamectin (ABM), a naturally fermented product of Streptomyces avermitilis, is applied to pest control in livestock and agriculture fields. The aim of the
separation factor RL Eq. (6) serves to categorize the isotherm type, where RL = 0, 0 < RL < 1, and RL > 1 correspond to irreversible, favorable, and unfavorable isotherms, respectively. The Freundlich isotherm equation involves the adsorption capacity Kf and (n), Eq. (...
with an absolute quantitation method (the primers are listed in Additional file3). Standard curves were established using plasmids with known copy numbers, allowing for the calculation of vector genome copy numbers based on this standard curve. The formula used for the copy number calculation was:...
Transcript detection and dropout frequency calculation For the comparison of transcript detection sensitivity between TARGET-seq and TARGET-seq+ (Figures S1G and S1H), data were first downsampled to 5 × 105 reads per cell to remove differences due to unequal sequencing depth. The number of genes...
(ChlSab1.1) were annotated using the Ensembl release 93 gene model. Abundance analysis was performed using our established RNA-Seq workflow in Partek Flow, which allowed calculation of transcript-level expression of a gene’s isoforms for alternative spliced transcripts [60,61]. Transcript abundances...
The raw kinase transcript read counts were then normalized with a calculation of reads per kilobase of exon model per million mapped reads (RPKM) (Mortazavi et al., 2008). The value of “N” (total number of mappable reads) in the RPKM formula was defined as the total number of aligned...
The A. argyi leaves were dried in the shade. The roots and stems were killed at 105 °C for 15 min and then dried at 65 °C until a constant weight was reached. The calculation formula for the dry matter distribution index is as follows [18]: 𝑃𝐼𝑖=𝑊𝑖/∑𝑊𝑖PIi=Wi...
In this formula, Δa is the relative increase in plant height, an is the plant height at the current measurement, and an−1 is the plant height at the last measurement. The aboveground enrichment coefficient reflects the ability of plants to absorb pollutants from the soil, and it was cal...