The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based ...
Fluorescence microscopy, with its molecular specificity, is one of the major characterization methods used in the life sciences to understand complex biological systems. Super-resolution approaches1,2,3,4,5,6can achieve resolution in cells in the range of 15 to 20 nm, but interactions between ...
of beleuchtungsstrahlung (7) on the fluorescence of stimulated\n(c) the sample, (2) in a field of view (17), the only part of the sample (2) repeatedly on a ortsaufl\\u00f6senden detector (5), so that a sequence is received.\n(d) from the image sequence by means of a kumu...
This chapter discusses the simple yet powerful principles that make it possible to neutralize the resolution-limiting role of diffraction in far-field fluorescence nanoscopy methods such as STED, RESOLFT, PALM/STORM, or PAINT. In a nutshell, feature molecules residing closer than the diffraction ...
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Design, building and use of a fluorescence recovery after photobleaching instrument D.L. Taylor, Y.-L. Wang (Eds.), Fluorescence Microscopy of Living Cells in Culture, Part B. Quantitative Fluorescence Microscopy: Imaging and Spectroscopy, 30, Academic Press, San Diego (1989), pp. 271-306 Go...
Image (A) and zoom-in image (B) from a slightly different field of view provided by Ian Clements, GE Healthcare. (Top) STED microscopy: Stimulated emission depletion microscopy The first technique to break the diffraction limit, STED microscopy...
1.4.8Super-resolution microscopy Super-resolution microscopyuses distinct properties of fluorescence to overcome the resolution limit of conventional microscopy [74]. This microscopy is used to visualise details of cellular structures enabling image-resolutions of 20–50 nm [71,75]. This technology emp...
Chromatin substructures fall into a resolution gap between EM and optical microscopy. ? Super-resolution fluorescence microscopy is well suited to access these substructures. ? We review new insights into chromatin structure gained by super-resolution imaging. ? We discuss technical developments in super...
摘要: Live cell fluorescence imaging of GFP labelled mitochondria of budding yeast cells using 4Pi-confocal microscopy is presented. This technique allows 3-5 times finer optical sectioning than confocal microscopy.关键词: Microscopy Fluorescence microscopy Three-dimensional microscopy ...