For successful infection of host cells and virion production, enveloped viruses, including Zika virus (ZIKV), extensively rely on cellular lipids. However, how virus protein–lipid interactions contribute to the viral life cycle remains unclear. Here, we
For successful infection of host cells and virion production, enveloped viruses, including Zika virus (ZIKV), extensively rely on cellular lipids. However, how virus protein–lipid interactions contribute to the viral life cycle remains unclear. Here, we
The full- length XistAR RNA sequence is B2.8 kb in length and is predicted to be non-protein coding through the Coding Potential Calculator algorithm29. Next, we mapped sequences in Xist exon 1 that may function as a promoter and enhancer of XistAR via luciferase reporter assays. In ...
Window size was determined using the SWATH variable window calculator tool (SCIEX) from summed total ion chromatograms (TIC) of library-generated samples. SWATH library data files were processed in Protein Pilot (v. 5.0) and searched against the UniProt C. jejuni NCTC11168 proteome (as above)...
ELISA was used to detect the potency titer of the anti-AjFoxO antibody. Specifically, 100 µL of 100 µg/mL rAjFoxO protein was coated onto 96-well flat-bottom microplates (Costar, USA) and incubated overnight at 4 °C. The wells were then washed three times with PBST and blocked ...
Protein was extracted by the hypotonic lysis protocol immediately after the cells were harvested post-transfection as was outlined in the above section. 0.5 ml of hypotonic lysis buffer was added to each sample per 100 × 200 mm tissue culture plate, with an additional 0.5 ml of hypotonic lysis...
For inverted Matrigel invasion assays38, Matrigel protein matrix (BD Bioscience) was allowed to polymerize in Transwell permeable inserts (Corn- ing Ltd) over a period of 60 min at 37 °C. Inserts were inverted, and cells seeded di- rectly onto the filter surface in complete growth ...