Next, we sought to explore whether macropinocytosis facilitates the survival of hypoxic HCC cells. To address whether extracellular proteins support hypoxic HCC cell growth, we employed BrdU assay and XTT assay to measure the cell proliferation of MHCC97L cells cultured in low-glutamine DMEM (0.2...
2C), there was no increase in multipolar cell population with no mitotic arrest in MDA-MB-468 cells (Supplementary Fig. S3A–C). Furthermore, TACC3 inhibition using BO-264 caused multipolar mitosis only in JIMT-1 and MDA-MB-231 cells with CA (Fig. 2F, G), but not in the non-CA...
To confirm the Biacore data, we used isothermal calorimetry (ITC) to investigate the interaction between RTA and the P-protein complexes in solution. Since RTA has a tendency to aggregate at high protein concentration, we used a low concentration of RTA in the cell and titrated human protein c...