Reporter Assay Protocol Materials to Be Supplied by the User ? luminometer ? siliconized polypropylene tube or small glass vial 6.A. Preparation of Luciferase Assay Reagent II Prepare Luciferase Assay Reagent II (LAR II) by resuspending the provided lyophilized Luciferase Assay Substrate in 10ml of...
Nano-Glo® Dual-Luciferase® Reporter Assay System Injectors ProtocolRELATED PRODUCTSNano-Glo® Dual-Luciferase Reporter Assay System Need Assistance Contact us Download Video: Closed Format: MP4 Open Format: Video Description The Nano-Glo® Dual-Luciferase® Re...
该内置的模板可在 Protocol Library 标签下的 Reporter Assay 中，或是在 SoftMax Pro 软件模板分享网站中找到 (www.softmaxpro.org)。筛选类应用 使用 SpectraMax Glo Steady-Luc Repoter 试剂盒对转染 pG l4 萤火虫荧光素酶的细胞平板进行多次读取，模拟报告基因检测筛选。细胞如前所述转染，在 96 孔板中以每孔...
10min 过后,使用 SpectraMax i3x 读板机内置的 S p e c t r a M a x G l o S t e a d y - L u cReporter 实验模板检测发光。该内置的模板可在 Protocol Library 标签下的 ReporterAssay 中,或是在 SoftMax Pro 软件模板分享网站中找到 (www.softmaxpro.org)。 筛选类应用 使用SpectraMax Glo...
Optimize your reporter gene assay experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs. View the relevant questions below: Having proble...
Luciferase Reporter Assay KitCatalog Number KA
of placental alkaline phosphatase for both secreted and membrane-bound forms of the enzyme. Our two kits allow a detection incolorimetry(using pNPP; detection at 405 nm) orchemiluminescence. Both kits are based on a “mix and read” assay protocol that is compatible withhigh throughput screening...
Cite this protocol Hutten, S., Dormann, D. (2022). Hormone-Inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases. In: Goldberg, M.W. (eds) The Nuclear Pore Complex. Methods in Molecular Biology, vol 2502. Humana, New York, NY. https://doi.org/...
signaling pathway analysis during gene transcription or translation levels. Assay kits are preferred over standalone reagents in cases where a validated protocol for assay development is available. Assay kits not only save time but also offer technological advantages, easy availability, and greater ...
6. Dual-Luciferase® Reporter Assay Protocol Materials to Be Supplied by the User • luminometer • siliconized polypropylene tube or small glass vial 6.A. Preparing Luciferase Assay Reagent II Prepare Luciferase Assay Reagent II (LAR II) by resuspending the provided lyophilized Luciferase Assay...