在有RELION的电脑上输入以下命令: relion_image_handler --i yangcong.mrc --o yangcongnew.mrc --angpix 1.07 --rescale_angpix 2.14 --new_box 400 有时候遇到奇数偶数问题,如果不成功,换成: relion_image_handler --i yangcong.mrc --o yangcongnew.mrc --angpix 1.07 --new_box 400 --force_header...
2. 打开volume viewer，显示voxel size（示例中为1.07），并查看其显示效果。3. 若要调整参数，如将pixel size更改为2.14，box设为400 pixel，可在RELION环境下执行以下命令：relion_image_handler --i yangcong.mrc --o yangcongnew.mrc --angpix 1.07 --rescale_angpix 2.14 --new_box 40...
relion_image_handler /usr/bin/relion_import /usr/bin/relion_localsym /usr/bin/relion_localsym_mpi /usr/bin/relion_mask_create /usr/bin/relion_merge_particles /usr/bin/relion_motion_refine /usr/bin/relion_motion_refine_mpi /usr/bin/relion_mrc2vtk /usr/bin/relion_particle_FCC /usr/bin/...
Image-processing software for cryo-electron microscopy relion.readthedocs.io/en/latest/ Topics guihpcmolecular-structurescudaimage-processingnvidiahigh-performance-computinggraphics-programmingctfregularizationcryo-emmicroscopylikelihoodmaximum-likelihoodoptimization-algorithmsrelionmolecular-biologyatomistic-modelsbiomolecular...
relion_image_handler --i J###.volume.map_half_A.mrc --new_box 288 --o J###new-box288.mrc Now I am using cryosparc-particles.star and J###new-box288.mrc in Relion to run the refinement using the command: which relion_refine_mpi --o Refine3D/job039/run --auto_refine --split...
bin/relion_image_handler: ELF 64-bit LSB pie executable, ARM aarch64, version 1 (GNU/Linux), dynamically linked, interpreter /lib/ld-linux-aarch64.so.1, for GNU/Linux 3.7.0, not stripped bin/relion_import: ELF 64-bit LSB pie executable, ARM aarch64, version 1 (GNU/Linux), dynami...
relion_image_handler /usr/bin/relion_import /usr/bin/relion_localsym /usr/bin/relion_localsym_mpi /usr/bin/relion_mask_create /usr/bin/relion_merge_particles /usr/bin/relion_motion_refine /usr/bin/relion_motion_refine_mpi /usr/bin/relion_mrc2vtk /usr/bin/relion_particle_FCC /usr/bin/...
The particles.star that does work does not contain a rlnRandomSubset column either. Here is what it contains: # RELION; version 3.0-beta-2 data_ loop_ _rlnCoordinateX#1_rlnCoordinateY#2_rlnClassNumber#3_rlnAutopickFigureOfMerit#4_rlnAnglePsi#5_rlnImageName#6_rlnMicrographName#7_rlnMagnific...
-- added image_handler... -- added localsym... -- added localsym_mpi... -- added maingui... -- added manualpick... -- added mask_create... -- added particle_polish... -- added particle_polish_mpi... -- added particle_reposition... ...
relion_image_handler /usr/bin/relion_import /usr/bin/relion_localsym /usr/bin/relion_localsym_mpi /usr/bin/relion_mask_create /usr/bin/relion_merge_particles /usr/bin/relion_motion_refine /usr/bin/relion_motion_refine_mpi /usr/bin/relion_mrc2vtk /usr/bin/relion_particle_FCC /usr/bin/...