Relative fold change in the mRNA levels of HIF-1α in D407 and ARPE-19 cell lines exposed to hypoxia in presence or absence of honokiol (A).Divya Teja VavilalaV. K. Chaithanya PonnaluriDebolina KanjilalMridul Mukherji
Real-time Q-PCR was used to analyze the relative expression levels of Frizzed 1-10 mRNAs in the mice ovaries at different estrous stages.The results showed that they had different expression patterns except Frizzed 8.Expressions of Frizzed 1 and 2 were the highest in proestrus and both were...
Real-time PCR for mRNA quantitation. Biotechniques. 2005;39(1):75–85. https://doi.org/10.2144/05391rv01. Article CAS PubMed Google Scholar VanGuilder HD, Vrana KE, Freeman WM. Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques. 2008;44(5):619–26. ...
Growth performance, carcass characteristics, hepatic enzyme activity, serum biochemical parameters, and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid pa...
2 15 s. Relative quantification of specific mRNA levels was analyzed using the cycle threshold (Ct) 2 − ΔΔCt method. Relative expression levels are normalized using the expression of GAPDH and shown in fold changes (lowest value = 1). Student's t-test (Po0.05) was used to determine...
Relative quantification of specific mRNA levels was analyzed using the cycle threshold (Ct) 2−ΔΔCt method. Relative expression levels are normalized using the expression of GAPDH and shown in fold changes (lowest value=1). Student’s t-test (P<0.05) was used to determine the significant ...
therelativechangeapplicationsoftwovariationsofthe2 CT methodthatmaybe usefulintheanalysisofreal-time,quantitativePCRdata. 2001ingeneexpressionwillsuffice.Forexample,stating ElsevierScience(USA) thatagiventreatmentincreasedtheexpressionof KeyWords:reversetranscriptionpolymerasechainreaction; genexby2.5-foldmaybemore...
Analysis of Relative Gene Expression Data Using Real METHODS25,402–408(2001)doi:10.1006/meth.2001.1262,available online at http://www.idealibrary.com on
Neuropsychopharmacology Figure 6 Gene and protein expression analysis using western immuno- blotting in the nucleus accumbens (NAc) and ventral tegmental area (VTA) of rats 24 h after salvA (0.0, 2.0 mg/kg). (a) TH and DAT mRNA levels are increased in the VTA 24 h after salvA (0.0, ...
The expression of galectin-1 mRNA was quantified by RT-PCR using β-actin as an internal standard for normalization. For ChIP assays, cells were grown under normoxia or treated with CoCl2 and then cross-linked and quenched. Subsequently, cells were lysed and sonicated, yielding 200–1000 bp DN...