Subsequently, extracts were centrifuged for 3 min at 6000 RPM (EBA20, Hettich Zentrifugen, Tuttlingen, Germany). The supernatant was made up to 3 mL with 40% methanol in a volumetric tube and subsequently filtered through 0.2 µm Teflon syringe filter (Spartan, 13/0.2 RC, Whatman, Germany...
The correlation between NMR data and gel rheology/stability was evaluated. The study indicated that (1) the gels greatly differed in the amounts of branched, crosslinked, and overall modified HA, with most of the HA being branched; (2) unexpectedly, the conventionally measured rheological ...