RCF is related to centrifuge speed setting (rpm) using the following equation: where "r", expressed in cm, is the radial distance from the center of the centrifuge head to the bottom of the tube. The following table gives recommended centrifuge RCF and time: Centrifugation RCF and Time* ...
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Fibroblast growth factor-2 (FGF2) has multiple roles in cutaneous wound healing but its natural low stability prevents the development of its use in skin repair therapies. Here we show that FGF2 binds the outer surface of dermal fibroblast (DF)-derived e
30μl of 10% Triton-X was added and the lysate centrifuged for 10 minutes at 20,000 rcf to separate debris. Samples were incubated with primary antibody at 4 oC overnight [anti-KDM5B (Novus Biologics 22260002)]. Samples were then precipitated with Dynabeads Protein G for 2 hr. The ...
An 0.2-ml volume of chloroform was added to each sample and shaken vigorously, and then samples were centrifuged at 12,000 relative centrifugal force (RCF) for 15 min at 4°C. The aqueous phase was transferred to a clean tube with 0.5 ml isopropanol and inverted to mix, and 2 µl ...
Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and imm
Bromodomain and extra-terminal domain (BET) proteins are therapeutic targets in several cancers including the most common malignant adult brain tumor glioblastoma (GBM). Multiple small molecule inhibitors of BET proteins have been utilized in preclinical
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates the development of new therapeutics, therapies, and prevention methods. Among the various therapeutic approaches, interventi
Lysates were then centrifuged for 20 min at 17,200 RCF at 4 °C to remove any cellular debris. The supernatant was collected, and lysate protein concentrations were determined by a BCA protein assay (Thermo Scientific Pierce, Waltham, MA, USA). Equal amounts of protein (15 to 20 μg ...
Chambers were incubated for 48 h aotn3t7h °eCloinwear5s%urCfaOce2 atmosphere. Cells on upper surface of were incubated for 3 h in 400 µl fresh membranes were removed with culture medium supplemented cotton swabs. Cells with MTT reagent. After incubation, culture medium was removed...