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(95%CI=19.2-82.0%) 14-20 days following the very first dose, 87.9% (95%CI=51.7-97.0%) 21-27 days after the very first dosage, and 96.0% (95%CI=82.2-99.1) 7 or even more days following the 2nd dose. Unvaccinated HCWs remain a concern even in the context of pandemic disaster....
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The biological purpose of PRUNE2 in CRC cell outlines had been examined using Cell Counting Kit-8, colony development, circulation cytometry and Transwell assay. Also, a mouse model ended up being set up to investigate the effect of PRUNE2 on metastasis of CRC cells. The phrase quantities of...
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Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labelled cRNA was synthesized (Enzo Bioarray High Yield RNA transcription Labeling Kit, Affymetrix), and hybridized to Affymetrix (Santa Clara, CA)...
and stored at −80 °C. Total RNA was extracted using an EZ-10 DNAaway RNA Miniprep kit (Bio Basic) according to the manufacturer’s protocol. Complementary DNA was synthesized using a Verso cDNA kit (Thermo Scientific) and used for quantitative real-time PCR (qRT-PCR). Primers were de...
Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labelled cRNA was synthesized (Enzo Bioarray High Yield RNA transcription Labeling Kit, Affymetrix), and hybridized to Affymetrix (Santa Clara, CA)...
RNA samples were collected at 6 h post-infection using RNeasy Mini Kit (Qiagen). Transcriptomic analyses were performed essentially as described. Total RNA (200 ng) from the various cell populations was reverse transcribed and fluorescently amplified using the Agilent Low-input Quick Amp single...