medical emergency kitfirst aid kit.FLIGHTPASSENGERSOXYGENINTRODUCTION: On -board medical emergencies are increasing. Different geographies have different legislation and requirements for medical emergency kits and first aid kits. A comprehensive review to compare the contents of both kits was conducted, ...
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The functional modules related to muscle contraction were the most downregulated modules associated with the exercise response gene list. For example, the largest over-represented module,Contractile fibre,contained 56 genes, which were mostly (44/56) downregulated (mean: −1.52-fold) in exercise rel...
(final 0.1% dimethyl sulfoxide, DMSO) was then added to the cells using the Bravo Automated liq- uid handling platform (Agilent technologies) (day 1), and cell viability was assessed with a CCK-8 kit (Cell Count- ing Kit-8, Dojindo, Kumamoto, Japan) after 72 h of incu- bation (...
Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labelled cRNA was synthesized (Enzo Bioarray High Yield RNA transcription Labeling Kit, Affymetrix), and hybridized to Affymetrix (Santa Clara, CA)...
After removal of LIF and the addition of appropriate factors to promote differentiation, ES cells lose their pluripotency and differentiate into a variety of cell types, including neurons, cardiac cells, skeletal muscle cells, and adipocytes [7], [8], [9]. Under these conditions, a significant...
Papillary thyroid carcinoma (PTC) is a common endocrine malignancy with low death rate but increased incidence and recurrence in recent years. MicroRNAs (miRNAs) are small non-coding RNAs with diverse regulatory capacities in eukaryotes and have been fre
Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labelled cRNA was synthesized (Enzo Bioarray High Yield RNA transcription Labeling Kit, Affymetrix), and hybridized to Affymetrix (Santa Clara, CA)...
b Contents of patchoulol in leaves significantly increased after MeJA treatment. The amount of patchoulol was first calculated comparing with external standard and then dividing by the fresh weight of the samples. Error bars indicate the SD of three biological replicates. ** indicates significant ...
The immunoprecipitated DNA was analyzed by real time PCR using the iQ™ SYBR Green Supermix kit from Bio-Rad and semi quan- titative PCR using the Gene Amp PCR core kit from Applied Biosystems. The primers for the MT-3 promo- ter were designed to span certain segments of the MT-3 ...