Jacobsson, Extensions to peak alignment using reduced set mapping: classification of LC/UV data from peptide mapping, Journal of Chemometrics 18 (2004) 465-473Åberg KM, Torgrip RJO, Jacobsson SP (2004) J Chemom 18:465–473Aberg KM, Torgrip RJO, Jacobsson SP (2004) J Chemom 18:465–...
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It dissects the peptide into 13-residue fragments, which first conveys the same advantage over our approach as discussed for MAMMOTH above. The algorithm then chops up the fragment into clusters. In mapping clusters the identity of amino acid (types, presumably) is enforced [50], an ...
peptides (Fig.2b). Verifying these results, we used radioactive methionine incorporation to measure mRNA translation and did not observe any differences between WT animals andppp-1mutants, whilersks-1/S6K mutants showed a drastic reduction (Fig.2c). To gain a deeper understanding of potential ...
Protein and peptide expression values were compared as log2 data with two-tailed t-test (two groups). Means of two groups were compared using two-tailed, unpaired t-test. If the F-test defined significantly different variances, the Mann–Whitney test was performed instead of the t-test. ...
(this study and16) are estimated to be in the near-micromolar range; indeed, given the likely further concentration of peptide at the synapse, synaptic 7B2 concentrations may reach levels many times this. Interestingly, CSF 7B2 concentrations have been reported to decline during normal aging32. ...
Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC. Biotechnol Bioeng. 2012;109:2349–56. Article CAS PubMed Google Scholar Zhang W, Xie H, He Y, Feng J, Gao W, Gu Y, Wang S, ...
mann m. accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed maxlfq. mol cell proteomics. 2014;13:2513–26. article cas pubmed pubmed central google scholar mortazavi a, williams ba, mccue k, schaeffer l, wold b. mapping and qu...
3.3. Proteomics mapping of different stages of pre- and post- HFrEF with XSB treatment A total of 2.5 μg FASP digested peptides from cardiac tissues (corresponding to 5 μg of initial heart proteins) was used for each mass spectrometry experiment. Three biological replicates were conducted...