Provided herein are methods of processing a polypeptide or protein for analysis, e.g., peptide mapping analysis by mass spectrometry. In exemplary embodiments, the method comprises incubating a digested sample at a mildly acidic pH and/or in the presence of a chaotrope, wherein the digested ...
Tandem mass spectrometry (MS/MS)-based strategies are presently the method of choice for large-scale protein identification due to its high-throughput analysis of biological samples. With database sequence searching method, a huge number of peptide spectra generated from MS/MS experiments are routinel...
Application note:Comparison of alternative approaches to trypsin protein digestion for reproducible and efficient peptide mapping analysis of monoclonal antibodies Application note:High-precision, automated peptide mapping of proteins Application note:Easy, fast and reproducib...
BiopharmaLynx decreases the expense and time-to-market of protein-based drugs by reducing data analysis time for peptide mapping studies. This software offers fast data processing and an easy-to-use interface that saves time and allows scientists to engage in high-value tasks. ...
Finally, to test the off-targeting propensity of our peptide-guided uAbs, one dimensional liquid chromatography-tandem mass spectrometry (1D-LC-MS/MS) analysis was performed on total proteins harvested from cells overexpressing β-catenin, with or without treatment with the uAb candidates, with ~67...
Since the flexible conformation and variable residue composition in a loop region may provide additional specificity for recognition of Abs, identification of UPMs may further aid in epitope mapping as well as peptide antigen design. Table 3 The sequences of human protein families for RMA analysis ...
Peptide Mapping for Biotherapeutics ebook资料库号码: LITR135132554 部件代码: 720008278EN 作者: Waters 消息来源: Waters 内容类型: 文献 内容子类型: 宣传手册 摘要This eBook provides an overview of important aspects of the Strategies for Simplifying Protein Digestion by focusing Peptide ...
symposium‘NewImpuritiesControl:SettingSpecificationsforAbbreviationsused:AAA,aminoacidanalysis;AES,atomicemission spectrometry;API,activepharmaceuticalingredient,syn.drugsubstances;CEP, AntibioticsandSyntheticPeptides’[3].AlthoughnotintendedforCertificateofSuitabilitytotheMonographsoftheEuropeanPharmacopoeia; ...
In vivosiRNA targeting and analysis Transgenic CAG-GFP mice were purchased from The Jackson Laboratory (stock #006567). Brain injuries were done as described above. Peptide-conjugated and siRNA-loaded PSiNPs (300 μg) were administered twice via tail-vein injections at 6 and 24 h post in...
Identification of HLA peptides as tumor antigens and candidates for immunotherapy is complicated by the difficulty of detection of their presentation on tumor cells. HLA peptidome analysis is limited by the yield of immunoaffinity purified HLA molecules and by the technical limitations of the chromatogr...