The recycling endosome (RE) is one of the membranous organelles involved in the protein recycling pathway. To determine whether RE is involved in the internalization of β 1 -AR upon ligand stimulation, we evaluated the localization of β 1 -AR after stimulation with a β-agonist, isoproterenol...
We introduced fluorescent markers of the Golgi (GRIP::mCherry), early endosome (RAB-5::mCherry) and RE (RAB-11::mCherry) into ccm-3( À / À ) (Fig. 3a,e–g). Ablation of ccm-3 did not affect early or late endosomes (RAB-7::mCherry), but caused a severe dispersal of ...
To test if autophagy affects the endocytic trafficking of GluA2, we assessed the colocalization of GluA2 with EEA1 (an early endosome marker), LAMP1 (a late endosome marker), or transferrin. Hippocampal neurons (DIV 17) were incubated with the GluA2 antibody alone or along with transferrin....
7A). It was reported that PKC activation led to translocation of membrane proteins such as epidermal growth factor receptor to the pericentrion [33], we therefore performed co-localization analysis of OATP1B1 and Rab11, a marker of recycling endosome [34]. As shown in Fig. 7B, in the ...
The method measures transport of transferrin, a marker for endocytosis/recycling, from an endosome‐enriched donor fraction to immunoisolated Rab11‐positive acceptor recycling endosomes. Transfer of acridinium‐labeled transferrin is detected by a highly sensitive chemiluminescence reaction using a ...
We introduced fluorescent markers of the Golgi (GRIP::mCherry), early endosome (RAB-5::mCherry) and RE (RAB-11::mCherry) into ccm-3( À / À ) (Fig. 3a,e–g). Ablation of ccm-3 did not affect early or late endosomes (RAB-7::mCherry), but caused a severe dispersal of ...
Rab11 regulates recycling through the pericentriolar recycling endosome J. Cell Biol., 135 (1996), pp. 913-924 View in ScopusGoogle Scholar Wagner et al., 2012 C.S. Wagner, J.E. Grotzke, P. Cresswell Intracellular events regulating cross-presentation Front. Immunol., 3 (2012), p. 138 ...
Immunofluorescent staining revealed that most Crb3 is colocalized with the recycling endosome marker Rab11. These findings show that Crb3 may promote colon cancer cell migration by regulating the expression of GSLs on the cell surface... H Iioka,K Saito,E Kondo - 《Biochemical & Biophysical Re...
On the other hand, the recycling of TFR which is recycling endosome dependent was clearly down-regulated by siRNAs to Rab11 but not Vps35. We concluded that Jag1a and TFR used different pathways for their recycling and SNX17 regulated the recycling of Jag1a through a Vps35 but not Rab11-...
complex, can also directly interact with each other24, possibly by obtaining a closed post-fusion conformation of HOPS following late endosome-lysosome fusion45,46. In analogy herewith, a closed post EE-EE fusion conformation of the CORVET complex could precede the direct interaction of Vps3 and...