Recombinant protein production in plants: challenges and solutions. Methods Mol Biol. 2012; 824 :469–481. doi: 10.1007/978-1-61779-433-9_25. [ PubMed ] [ Cross Ref ]Hood EE, Requesens DV: Recombinant protein production in plants: challenges and solutions. Methods Mol Biol 2012, 824 : ...
The large-scale production of recombinant proteins in living cells or organisms; frequently applied to the use of crop plants or domestic animals as expression hosts because of the allusion to agriculture. HETEROLOGOUS In the context of this article, a gene or protein that is not derived from th...
The large-scale production of recombinant proteins in plants is limited by relatively low yields and difficulties in extraction and purification. These problems were addressed by engineering tobacco plants to continuously secrete recombinant proteins fro
Despite these and other attracti ve features, it is interesting that plants have not been llsed as hosts for recoJllbinant protein production on a cOlnrnercial scale. The reasons for this include: I. Concern about the environluental safety of plants producing distinctly non-plant producls 2...
Purification of soluble CD14 fusion proteins and use in an electrochemiluminescent assay for lipopolysaccharide binding:Burkhardt, M. et al., Protein Expr Purif. 2007 Jan;51(1):96-101 [Abstract] Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor:Baxter SC et al., ...
There have been many attempts to produce recombinant proteins of industrial importance in plants over the years. Plants have numerous advantages that make them a better option compared to other hosts that are normally used. These include the high scalability for mass production while using transgenic...
In the present study, we produced a recombinant, active SWO from T. harzianum using a prokaryotic host and confirmed its potential synergistic role in biomass degradation. Our work paves the way for further studies evaluating the structure and function of this protein, especially regarding its use...
P. pastoris has the capacity to produce large quantities of protein with eukaryotic processing. Expression is controlled by a methanol-inducible promoter, which allows a biomass-generation phase before protein production is initiated. The target protein is secreted directly into a protein-free mineral ...
in mammalian cells versus other hosts such as bacteria, plants and yeast. Recently, the productivity of mammalian cells cultivated in bioreactors has reached the gram per liter range in a number of cases, a more than 100-fold yield improvement over titers seen for similar processes in the mid...
We use the model to evaluate the secretion of various recombinant proteins and predict engineering targets for improving their production. The model represents a significant advancement in terms of enabling more rational design of yeast cells to be used for recombinant protein production, while further...