Hood EE, Requesens DV: Recombinant protein production in plants: challenges and solutions. Methods Mol Biol 2012, 824:469-481.Hood EE, Vicuna Requesens D (2012) Recombinant protein production in plants: challenges and solutions. In: Lorence A (ed) Methods in molecular biology: recombinant gene ...
The large-scale production of recombinant proteins in plants is limited by relatively low yields and difficulties in extraction and purification. These problems were addressed by engineering tobacco plants to continuously secrete recombinant proteins from their roots into a simple hydroponic medium. Three ...
The large-scale production of recombinant proteins in living cells or organisms; frequently applied to the use of crop plants or domestic animals as expression hosts because of the allusion to agriculture. HETEROLOGOUS In the context of this article, a gene or protein that is not derived from th...
Despite these and other attracti ve features, it is interesting that plants have not been llsed as hosts for recoJllbinant protein production on a cOlnrnercial scale. The reasons for this include: I. Concern about the environluental safety of plants producing distinctly non-plant producls 2...
Purification of soluble CD14 fusion proteins and use in an electrochemiluminescent assay for lipopolysaccharide binding:Burkhardt, M. et al., Protein Expr Purif. 2007 Jan;51(1):96-101 [Abstract] Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor:Baxter SC et al., ...
专利名称:RECOMBINANT PROTEIN PRODUCTION 发明人:ホワイト,ロバート 申请号:J P 2018534 839 申请日:20170109 公开号:JP2019500875A 公开日:20190117 摘要:The disclosure relates to recombinant protein expression systems comprising genetically modified cells wherein the cells are transformed or transfected with...
•App Note:Recombinant protein expression using a baculovirus-insect cell system •Review:Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products •Podcast:Producing challenging proteins in the golden age of protein engineering ...
Our data demonstrate that Delta XT/FT N. benthamiana plants can be engineered toward the production of recombinant IgA1 with defined human type Nand O-linked glycans. 展开 关键词: N-glycosylation O-glycosylation antibody glyco-engineering monomeric IgA plant-made pharmaceuticals protein glycosylation ...
P. pastoris has the capacity to produce large quantities of protein with eukaryotic processing. Expression is controlled by a methanol-inducible promoter, which allows a biomass-generation phase before protein production is initiated. The target protein is secreted directly into a protein-free mineral ...
SUBSTANCE: method involves the realization of recombinant DNA expression in plants. Indicated recombinant DNA causes the destruction of nutrient structure for nematodes or expression of indicated recombinant DNA inhibits the formation of nutrient structure for nematodes. Indicated recombinant DNA involves: 1...