Wingfield PT. Production of recombinant proteins. In: Coligan JE, Dunn BM, Speicher DW, Wingfield PT, Ploegh HL, editor. In Current Protocols in Protein Science. Vol. 1. New York: John Wiley & Sons; 2000. pp. 5.0.1–5.3.18.
The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic cells. In this expression system, induction of heterologous protein is achieved by increasing ...
Cultivated mammalian cells have become the dominant system for the production of recombinant proteins for clinical applications because of their capacity for proper protein folding, assembly and post-translational modification. Thus, the quality and efficacy of a protein can be superior when expressed in...
E. coliexpression systems have been the preferred option for producing many recombinant proteins in high quantities and low production costs [1–6]. Among the underlying reasons for such a widespread use ofE. coliis the availability of a variety of strong inducible promoters [7]. The promoters ...
Escherichia coli, one of the most efficient expression hosts for recombinant proteins (RPs), is widely used in chemical, medical, food and other industries. However, conventional expression strains are unable to effectively express proteins with complex structures or toxicity. The key to solving this...
Production of recombinant proteins by yeast cells Pichia pastorisExpression vectorFermentation optimizationPost-translational modificationYeasts are widely used in production of recombinant proteins of medical or ... E ?Elik,P ?Al?K - 《Biotechnology Advances》 被引量: 269发表: 2012年 Production of lar...
Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium. Zang, M. et al.; " Production of Recombinant Proteins in Chinese Hamster Ovary Cells Using a Protein-Free Cell Culture Medium "; 1995; Biotechnology; ... C Gandor,F Messi,F Asselbergs...
The large-scale production of recombinant proteins in living cells or organisms; frequently applied to the use of crop plants or domestic animals as expression hosts because of the allusion to agriculture. HETEROLOGOUS In the context of this article, a gene or protein that is not derived from th...
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience. In most cases production of heterologous proteins in Escherichia coli K12 strains has remained an empirical exercise in which differen...
The present invention relates to recombinant proteins encoding at least two antigenic epitopes joined by flexible hinge domains. Proteins of this design enable presentation of each epitope to the immu