Renaturation, activation, and practical use of recombinant duplex-specific nuclease from Kamchatka crab. Biochemistry (Mosc.) 71, 513-519.Anisimova,V.E., Rebrikov,D.V., Zhulidov,P.A., Staroverov,D.B., Lukyanov,S.A. and Shcheglov,A.S. (2006) Renaturation, activation, and practical ...
Duplex-specific Nuclease, Crab, recombinant, Solution Boiling point Melting point Density MSDS Formula Use,Duplex-specific Nuclease, Crab, recombinant, Solution 融点、価格、蒸気圧、沸点、毒性、比重、沸点、密度、分子式、分子量、物理的な性質、毒性.
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Berk AJ: Characterization of RNA molecules by S1 nuclease analysis. Methods Enzymol. 1989, 180: 334-347. Article CAS Google Scholar Shishido K, Ando T: Estimation of the double-helical content in various single-stranded nucleic acids by treatment with a single strand-specific nuclease. Biochim...
Deletions of dsDNA can be prepared by the BAL 31nuclease. Theincubation timerequired to produce the desired deletion can be estimated using the equation Mt=M0−2MnVmaxt[Km+(S)0] whereMtis the molecular weight of the duplex DNA aftertminutes of incubation,M0is the original molecular weight ...
Secondly, protein-based bioPROTACs exhibited no loss in activity for several weeks, are not susceptible to nuclease activity, and are amenable to typical storage conditions. For Ras-targeting, we found that the general trend for degradation rate was, in increasing order: siRNA <DNA <mRNA <...
Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates.###Determination of neutrophil antigen HNA-3a and HNA-... Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5'-end and the corresponding siRNA duplexes have been prepared using phosphoramidi...
(representing the 83-nt RNA from the 3'-end of JEV genome) were obtained by PCR with Vent DNA polymerase and the specific primers for 3'(+)UTR RNA (5'-TAATACGACTCACTATA GCTAGTGTGATTTAAAGTA-3' and 5'-AGATCCTGTGTTCTTCC-3'), 3'(-)UTR RNA (5'-AGAAGTTTATCTGTGTG-3' and 5'...
Alternatively, homology can be determined by hybridization of polynucleotides under conditions which form stable duplexes between homologous regions, followed by digestion with single-stranded-specific nuclease(s), and size determination of the digested fragments. Two DNA, or two polypeptide sequences ...
The insert DNA is then digested with the same two restriction enzymes thereby having two dissimilar DNA ends that correspond to a specific orientation in the target insertion site. By following this procedure, the insert DNA only binds to the target sequence in one orientation. ...