DSN(Duplex-specific nuclease)能够选择性降解双链DNA 和 DNA-RNA杂交体中的DNA,但对单链核酸分子和双链RNA几乎没有活性。DSN可以 区分10-12 bp的DNA-DNA双链单核苷酸错配,当dsDNA大于10bp、DNA-RNA大于15 bp时DSN能产生切割活性。其广泛用于全长cDNA富集后均一化操作、基于二代测序(NGS)的转录组文库构建,SNP...
Duplex-specific nuclease-mediated bioanalysisduplex-specific nucleasemiRNAdetectionnormalizationbiosensorfluorescence assayDuplex-specific nucleases (DSNs) are promising tools for bioanalysis because of their unique ability to cleave DNA within duplexes while keeping a single strand intact. There is prevalent ...
请问Duplex-specific nuclease (DSN)主要是怎样剪切DNA的，它能否剪掉G四连体呢？谢谢！
Duplex-specific nuclease (DSN) is an enzyme that, at high temperatures, degrades duplex DNA in preference to single-stranded DNA. DSN treatment has been successfully used to normalize the relative transcript abundance in mRNA-enriched cDNA libraries from eukaryotic organisms. In this study, we ...
An application of duplex-specific nuclease (DSN) normalization technology to whole-genome shotgun sequencing of genomes with a large proportion of repetitive DNA is described. The method uses a thermostable DSN from the Kamchatka crab that specifically hydrolyzes dsDNA. In model experiments on human ...
The present invention provides a thermostable duplex-specific nuclease (DSN) and a method for digesting a nucleic acid using said DSN, namely, a protein having a Brachyura-derived duplex-specific nuclease activity, a gene encoding for said protein, a recombinant vector comprising said gene and a...
Using degenerative primers and the rapid amplification of cDNA ends (RACE) procedure, we cloned the Duplex-Specific Nuclease (DSN) gene from the hepatopancreas of the Kamchatka crab and determined its full primary structure. We also developed an effective method for purifying functional DSN from ...
A novel recyclable surface-enhanced Raman spectroscopy platform with duplex-specific nuclease signal amplification for ultrasensitive analysis of microRNA 155microRNARecyclabilityDuplex-specific nuclease signal amplification (DSNSA)Fe3O4@PDA/PtSurface-enhanced Raman scattering (SERS)...
DSN(Duplex-specific nuclease)能够选择性降解双链DNA 和 DNA-RNA杂交体中的DNA,但对单链核酸分子和双链RNA几乎没有活性。DSN可以精确区分10-12 bp的DNA-DNA双链单核苷酸错配,当dsDNA大于10bp、DNA-RNA大于15 bp时DSN能产生切割活性。 产品参数 质量标准 ...
Nuclease activity was terminated through the addition of equal volume of 10mM EDTA and 95掳C incubation for 2min. Following DSN treatment, 21 of 30 alleles within the known profile exhibited some improvement in peak height balance. The findings obtained support the potential use of DSN treatment ...