美国加利福尼亚大学Stephen C Kowalczykowski等在《Nucleic Acids Research》发表文章:Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon χ recognition,提出了新见解。作者切断了RecBhCD 和 RecBn 之间的系链,发现仍能重建野生型 RecBCD 的活性,也可以...
RecBCD enzyme, also called exonuclease V (EC 3.1.11.5), is unusual among nucleases in its requirement for ATP. This large, three subunit enzyme has multiple enzymatic activities and plays important roles in many aspects of DNA metabolism, including recombination, repair and replication. Although ...
What is the best exonuclease to remove chromosomal DNA during a plasmid prep? Which enzyme can distinguish between mitochondrial and genomic DNA and only remove gDNA? Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations? Does NEB have an equivalent to Lucig...
RecBCD enzyme is a bipolar DNA helicase. Nature 423:893–897 - Dillingham, Spies, et al. - 2003 () Citation Context ...so, the E. coli RecBCD complex has been reported to contain bipolar enzyme activity, where the RecB and RecD components of the complex unwind DNA in the 3-to-5...
The Escherichia coli RecBCD enzyme is a powerful helicase and nuclease that processes DNA molecules containing blunt double-strand DNA end. Mutants deprived of RecBCD enzyme functions are extremely sensitive to DNA-damaging agents, poorly viable and severely deficient in homologous recombination. Remarkab...
RecBCD enzyme is the main component of the RecBCD recombination pathway. It is a multi-subunit enzyme which is a highly processive bipolar helicase and ATP-dependent ds- and ssDNA exonuclease which acts on linear DNA substrates. RecBCD enzyme alone converts double-strand ends into RecA–ssDNA ...
Cooperation of two motors was envisioned as being responsible for the fast enzyme translocation. The pause at χ was attributed to the time required for a χ-induced conformational change. In this model, the reduced rate after χ is a consequence of inactivation of one of the motors. Thus, ...
The RecBCD enzyme of Escherichia coli participates in several aspects of DNA recombination and repair. It is essential to the main pathway of genetic homologous recombination, where it contributes to the exchange of genetic material between homologous DNA molecules (i.e. conjugal recombination), and...
Repair of double-strand DNA breaks by homologous recombination can be carried out in bacteria by RecBCD, AddAB and AdnAB. Here, Dale Wigley discusses recent insights into how these complexes mediate repair, as well as their evolution. In bacteria, the pr
RecBCD enzyme facilitates loading of RecA protein onto ssDNA produced by its helicase/nuclease activity. This process is essential for RecBCD-mediated homo... SC Kowalczykowski - 《Molecular Cell》 被引量: 144发表: 2006年 Inhibition of the recBCD-dependent activation of Chi recombinational hot ...