- 2003 () Citation Context ...se and RecA loading activities of the RecBCD enzyme, for which its proper interaction with the RecB and RecC subunits is crucial (4, 5, 50, 51). Also, the RecD subunit contains a fast helicase domain =-=(16, 47)-=-. Since the RecBC(D) enzyme is...
The purified RecBCD holoenzyme has a native molecular mass of approximately 330 kDa, indicative of a heterotrimer subunit assembly. The RecB, RecC, and RecD subunits can associate in vitro to give nuclease, helicase, ATPase, and Chi-specific endonuclease activities which are indistinguishable from ...
Component of the enzyme; Importance of RecD enzyme for nuclease activity; Distribution of recombination around special sites on the chromosome.AmundsenSusanK.TaylorAndrewF.SmithGeraldR.EBSCO_AspGeneticsA domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli Rec...
gam-dependent radioresistance is observed in recB+ recD+ recD+ but not in recB+ recC+ recD− cells. It is suggested that Gam protein interacts specifically with the RecD subunit of RecBCD enzyme; the RecBC complex probably retains its activity in the presence of this viral protein.N....
RecBCD enzyme is the main component of the RecBCD recombination pathway. It is a multi-subunit enzyme which is a highly processive bipolar helicase and ATP-dependent ds- and ssDNA exonuclease which acts on linear DNA substrates. RecBCD enzyme alone converts double-strand ends into RecA–ssDNA ...
The RecD Subunit of the RecBCD Enzyme from Escherichia coli is a single-stranded DNA-dependent ATPase. J. Biol. Chem. 272:10072-10079.Chen, H.W., Ruan, B., Yu, M., Wang, J., and Julin, D.A. (1997). The RecD subunit of the RecBCD enzyme from Escherichia coli is a single-...
The RecBCD enzyme is a multifunctional protein complex essential to the main pathway of homologous recombination in Escherichia coli (1, 2). The holoenzyme, composed of the RecB, RecC, and RecD subunits, possesses DNA-dependent ATPase, helicase, and nuclease activities (3., 4., 5., 6., 7...
This strand is then channeled through the enzyme to the χ-recognition site residing in the RecC subunit. Afterward, the strands exit the enzyme in proximity to the active site of the nuclease domain located in the C terminus of the RecB subunit. Because the channel for the 3′-terminated ...
u.v.-irradiation of the complex cross-linked the RecB subunit to the 3′ terminated strand and the RecC and RecD subunits of the 5′ terminated strand. Studies using pyridoxal 5-phosphate, an inhibitor of the RecBCD enzyme, corroborated the cross-linking studies and revealed a conformational ...
Chen HW, Radle DE, Gabbidon M, Julin DA. 1998. Functions of the ATP hydrolysis subunits (RecB and RecD) in the nuclease reactions catalyzed by the RecBCD enzyme from Escherichia coli. J Mol Biol 278: 89-104.Chen, H.W., Randle, D.E., Gabbidon, M., and Julin, D.A. (1998) ...