Common methods for detecting EPNs in soil samples include baiting with sentinel insects, direct observation of extracted nematodes, or use of species-specific primer-probe combinations using qPCR. Less well studied is the use of high throughput sequencing (HTS), which has tremendous potential to ...
Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and...
Are there high-throughput options for real-time PCR that will allow me to scale up my experiments? Yes, TaqMan Array plates are 96- and 384-well plates containing 8–384 assays (pre-spotted and dried down, all you have to do is add cDNA and master mix). Based on the number of sam...
while our understanding of treatment options for low-grade (LGSOC) or mucinous (MUCOC) of ovarian malignancies is not developing as well. We describe here a functional precision oncology (fPO) strategy in epithelial ovarian cancers (EOC), which involves high-throughput...
Due to the low-cost of the MinION sequencing device (~$1000), total equipment costs are low relative to NGS sequencing. However, per-run costs can be as high as ~$625 ($475/flow-cell + ~$150/reagents). Assuming re-use, per-run costs are estimated to be ~$387.5 ($237.5/flow...
• a wide dynamic range for detection • a low per-sample experiment cost. • high sample throughputs The major advantages of digital PCR include: • no reliance on standard curves and reference samples; • high tolerance to biological and sample prep inhibitors; ...
DNA sequencing was also used to distinguish the prototypic HAdV type 14p (strain De Wit) from newly emerged type 14p1. Adenovirus type 14p1 has been associated with severe disease in Europe and the North America [2–5]. While the hexon HVR7 sequences obtained in this study share 100% id...
Introduction Real-time PCR (qPCR) has become the definitive approach to quantify gene expression and corroborate high-throughput microarray results in both basic sciences and clinical research. Because of the sensitivity of qPCR to subtle experimental varia- tions, a list of guidelines, known as ...
Operating tests in high-throughput settings under severe pressure is error prone, thus the robustness of the operational procedures is crucial including positive and negative controls and the careful review of results. The quality of the sample is also crucial and it should be taken into ...
Deep RNA sequencing (RNA-seq) has become a powerful tool in high-throughput transcriptomic studies with its high resolution, large data set, and sensitivity. Recently, this technique has been used to identify novel reference genes for model systems such as the human cell cultures, zebrafish, mice...