Real-time PCR amplification techniques are currently used to determine the viral load in clinical samples for an increasing number of targets. Real-time PCR reduces the time necessary to generate results after amplification. In-house developed PCR and nucleic acid sequence-based amplification (NASBA)...
Digital PCR counts individual molecules for absolute quantification Digital PCRworks by digitizing a sample into many individual real-time PCR reactions; some portion of these microreactions contain the target molecule (positive) while others do not (negative). Following PCR analysis,...
A quantitative real-time PCR assay was developed to measure the proviral load of human T-lymphotropic virus type I(HTLV-I) in peripheral blood.The technology utilizes special primers and Taqman MGB fluorescence probe to measure amplification products from the gag-pro-pol polyprotein gene of HTLV...
No, though comparing the intensity of the amplified band on a gel to standards of a known concentration can give you 'semi-quantitative' results. Applications Absolute quantification of viral load Absolute quantification of nucleic acid standards Absolute ...
The aim of this study was to isolate and analyse single cells using a novel platform, the PanelChip™ Analysis System, which includes 2500 microwells chip and a digital real-time polymerase chain reaction (dqPCR) assay, in comparison with a standard PCR (qPCR) assay. Through the serial ...
The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in se...
Quantitative PCR (qPCR), also called real-time PCR or quantitative real-time PCR, is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction.
Quantification using real-time PCR technology: applications and limitations. Real-time PCR has become an invaluable tool in molecular diagnostics, but the wide use of real-time PCR methods has also highlighted some of the critical p... D Klein - 《Trends in Molecular Medicine》 被引量: 1479发...
The present study was undertaken to evaluate cytomegalovirus (CMV) load in carious lesions in children with severe early childhood caries (S-ECC) and compare it with caries-free (CF), healthy children, by quantitative real-time polymerase chain reaction (PCR). Materials and methods Dental plaque...
quantitativePCRtechniquesincluded:(i)ensuringthat timePCR.Wehaveassembledninearticlesfromlead- thePCRwaswithinthelinearrangeofamplification ersinthefieldrangingfromquantificationofmRNA (thatportionwherethePCRsignalisdirectlypropor- andviralDNAtotheamplificationanddetectionof tionaltotheinputcopynumber)and(ii)findinga...