Real-time PCR instrument detection system workstation for Clinical Analytical Instruments Fluorescence Detection Long Life LED lamps Excitation CCDs Detection ≥1 copy Sensitivity F1:FAM,SYBR,Green F2:VIC,HEX,JOE,CY3,NED Suitable Fluorescent Dyes Specifications: Sample Block Capacity 48 wells...
引物的3’端的△G值过高,容易在错配位点形成双链结构并引发DNA聚合反应。 7、引物二聚体及发夹结构的能值过高(超过4.5Kcal/mol)易导致产生引物二聚体带,并且降低引物有效浓度而使PCR反应不能正常进行。 8、对应物的修饰一般是在5’端...
Working principle:The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity MPPC unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with m...
Heal Force Real Time PCR Principle-X320 2 Pages Heal Force Clean Bench Laminar Flow 2 Pages Clean bench/AlphaClean 1300 12 Pages Cytotoxic cabinet 20 Pages Heal Force Types of Biosafety Cabinets 18 Pages CO2 Incubator 6 Pages Heal Force Biological Safety Cabinet ...
Real-Time PCR 實驗配置 7500 相關耗材及實際儀器上機操作說明 7500 2.0軟體操作及結果分析說明 StepOne 2.1 軟體上機分析說明 Part I StepOne 2.1 軟體上機分析說明 Part II 7900 HT 相關使用耗材及儀器上機操作說明 Real-Time PCR 原理 Real-Time PCR 實驗設計 Primer & TaqMan Probe設計方案 Primer...
Biobase real time PCR PCR machine/qPCR Introduction: Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexibl...
引物设计原则(Principle of real-time quantiation PCR primer )(1)|of|pcr|primer|real-time|quantiation1、引物的长度一般为15-30bp,常用的是18-27bp,但不应大于38,因为过长会导致其延伸温度大于74°C,不适于TaqDNA聚合酶进行反应。2、引物序列在模板内应当没有相似
A schematic of the working principle of SG I in real-time PCR Full size image However, since SG I recognizes dsDNA in a nonspecific manner, nonspecific amplification products cannot be differentiated. Optimization of primers and template is therefore essential. First, the primers should be designe...
Fluorescence Real-Time Quantitative Gene Amplification Instrument PCR, Find Details and Price about Portabl PCR Thermal Cycler Thermal Cycler in PCR Machine from Fluorescence Real-Time Quantitative Gene Amplification Instrument PCR - Zhengzhou Laboao Ins
The kit adopts the principle of real-time fluorescent quantitative PCR technology, designs specific primers and probes for (ORF1ab/N gene), and combines them with real-time fluorescent quantitative PCR instrument to detect the nucleic acid of virus. So as to r...