Under his microscope, Jun Wu could see several tiny spheres, each less than 1 millimetre wide. They looked just like human embryos: a dark cluster of cells surrounded by a cavity, and then another ring of cells.But Wu, a stem-cell biologist at the Un... ...
Animals were cared for and maintained under the Guidelines of Laboratory Animals of Fudan University. All experiments involving animals or animal products were approved by the Animal Ethics Committee of Fudan University, China (approval No. 2021JSCHEM-020). The human research was approved by Ethics ...
The perfusion system included the microscope stage chamber MS4 (Digitimer) connected through the female luer lock (Drifton, LF31) to the Original Perfusor®tubing line (B. Braun, 8723060). The respective end of the Perfusor®line tubing was inserted into a 1 ml Stripette®(Corning, 4011...
Microscope. An Olympus FV1000-MPE laser scanning microscopy system based on an upright BX61-WI microscope was used in either confocal or multiphoton mode. A tunable MaiTai DeepSee Ti:sapphire laser was used for two-photon microscopy. Two air objectives (XL Fluor 2×/340 NA 0.14; UPlanFL ...
Additionally, an optical pH sensor and a microscope have been added in the platform for the real-time monitoring of the tissue. This model has the potential to study the absorption and metabolism of the drug along with the capacity to complete and optimize its toxicity assessment....
Under an Elsevier user license open archiveObjective Cardiac stem cell transplantation as a potential means of regenerating infarcted myocardium is currently receiving a great deal of interest. However, data on these endogenous cardiac precursors are primarily derived from animal studies, and their clinica...
7) This empirical treatment occurred 9 years before the first red cell was observed under a microscope by Antonie van Leeuwenhoek. The first successful husband to wife transfusion to treat a post-partum hemorrhage was reported in 1818 by Dr. James Blundel and was followed in 1840 by the ...
After histological processing, sections were analyzed to identify the presence of neutrophils, lymphocytes and plasma cells, eosinophils, macrophages and giant cells, as well as fibrous capsule and abscesses, by an examiner using light microscope. Kruskal- Wallis and multiple-comparisons...
The measuring point (embryo/embryonic axis) was located by microscope and the micro- optrode was adjusted to the point using a three dimensional motor. The oxygen fluxes were recorded every 10 seconds and measured for at least 5 minutes. Finally, the data of oxygen fluxes and the images ...
After fixation, the larvae were placed under the objective, and Holtfreter’s solution was used as the immersion medium of the objective. Before image acquisition started, we previewed the image and rotated the microscope slide manually to keep the larva horizontal or vertical in the FOV. Two-...