Lee课题组[127]使用了低能量的质子束照射LLC, HepG2和Molt-4三种肿瘤细胞表明, 照射后caspase的活性提高, 同时也证明了p38和JNK(不是ERK)能被ROS激活, 从而诱导细胞凋亡. 线粒体ROS(mitROS)在肿瘤细胞比正常细胞要高, 诱导Mn-SOD的增加. 肿瘤细胞中血红素转运蛋白HCP1表达增加, HCP1是一类转运卟啉类化合物的...
Most of the stem bnAbs we examined bound autoantigens; several showed staining of HEp-2 cells. A smaller proportion of the head-directed bnAbs were polyreactive. Gene usage did not correlate with autoreactivity. We suggest that complex foreign antigens may often have surface patches resembling ...
We hypothesized that IL-22 acts on microglia in the hippocampus to induce an inflammatory response in these cells by binding to IL-22Rα1 on their surface; this hypothesis was tested by double immunofluorescence staining for IL-22Rα1 and Iba-1. However, microglia act as innate immune cells...
This process produced serum autoantibodies to a breadth of self-antigens, leading to antibody deposition in the kidneys. Our data provide insight into the maturation of the self-reactive B cell response, contextualizing the epitope spreading observed in autoimmune disease. Graphical Abstract Download: ...
Haemophilus influenzae type b capsular polysaccharide vaccine in children: a double-blind field study of 100,000 vaccinees 3 months to 5 years of age in Finland. Pediatrics 60, 730–737 (1977). 5. Pon, R. A. Exploiting the Bacterial Surface: The Successfull Application of Glycoconjugate ...
As the recombination also results in those that are self-reactive, several mechanisms are in place to eliminate self-reactivity from the naive B-cell pool. At the pro-B- cell stage early in B-cell development VDJH recombination leads to expression of an antibody m-heavy (mH) chain. This ...
Here we report that a n[barbelow]a茂ve-like IgD[barbelow], IgMB cells (B) in humans that reach full maturity are negative for surface IgM expression, retaining only IgD, and are functionally attenuated. These Bcells typically make up 2.5% of peripheral blood B cells, have completely ...
Antibody Repertoire Analysis of COVID-19 Patients We first characterized the B cell response in COVID-19 using single-cell Ig gene sequencing of human mAbs (Figure 1A). From 10 COVID-19 patients with serum antibodies to the S1 subunit of the SARS-CoV-2 spike protein (Figure S1A; Table ...
(b) Proteins immunoprecipitated with mouse anti-human IgG (g chain- specific) antibody or normal mouse IgG from total lysates of HeLa cells were fractionated with 10% SDS-PAGE gel. The gels were either visualized with silver staining (upper panel) or blotted with anti-IgGg antibodies (lower ...
Celastrol also reduced cell viability in both H1299 and HepG2 cells in a dose-dependent manner (Figure 1B). Celastrol arrested cell cycle in both cell lines, and the G2-M phase ratio rose from 15 ± 1.6% to 41 ± 3.1% in H1299 cells and 15 ± 1.8% to 34 ± 3.5% in HepG2 ...