aChIP-reChIP assay in EL4 cells showing that SR1001 reduces RORa dependent (b) andRORc-dependent (c) recruitment of SRC2 and promotes the 芯片reChIP分析用试样在EL4表示的细胞SR1001减少SRC2的RORa依赖(b) andRORc依赖(c)补充并且促进[translate]...
Here we describe the ChIP 鈥搑eChIP assay that represents a direct strategy to determine the in vivo colocalization of proteins interacting or in close contact in a chromatinized template on the basis of double and independent rounds of immunoprecipitations with high-quality ChIP grade antibodies....
Re-ChIP (aka Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP) is a relatively new technique that enables sequential chromatin immunoprecipitations to be performed using two different antibodies so that you can assay for the simultaneous presence of two proteins or distinct histone modifications at...
Currently, intersection of H3K4me3 and H3K27me3 ChIP-seq tracks is the sole method to detect bivalent domains genome-wide. However, co-enrichment is not sufficient to establish whether H3K4me3 and H3K27me3 are indeed present on the same nucleosome and therefore co-localize. They may be presen...
While instructive, a major caveat of these reChIP experiments is the inefficient capture of the second co-localizing mark, due to harsh elution steps after the first ChIP. As a consequence, large amounts of input chromatin are required to obtain sufficient DNA for sequencing18. Furthermore, ...
In this chapter, we describe a simple and reliable method for the sequential ChIP assay. 展开 关键词: ChIP-re-ChIP Chemical cross-linking Chromatin immunoprecipitation (ChIP) Co-occupancy Polymerase chain reaction (PCR) Promoter/regulatory region occupancy Protein–protein interactions DOI: 10.1007/978...
(D) Metagene plots showing the average ChIP signal for H3K4me1, H3K27ac, and H3K27me3 for all of the Corin up- and downregulated genes in SU-DIPGXIII cells treated with DMSO or Corin. (E and F) Ranking of Corin-induced H3K27ac (E) and H3K27me3 (F) ChIP peaks to identify ...
examination of specific transcriptional or epigenetic regulators, or for the identification of cistromes, the ability to perform a sequential ChIP adds a higher level of power and definition to these analyses. In this chapter, we describe a simple and reliable method for the sequential ChIP assay....
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