RBL‐2H3 cellstheoretical calculationA fluorescent probe (Fluo-HMF) was developed via introduction of a furfural moiety into the fluorescein molecular skeleton, aiming at specially labeling cell membrane of mast cells. To illustrate its specificity, we designed and synthesized a series of fluorescent ...
参考文献 [1]Mast cells:an expanding pathophysiological role from allergy to other disorders[J].Preet Anand,Baldev Singh,Amteshwar Jaggi,Nirmal Singh.Naunyn-Schmiedeberg’s Archives of Pharmacology.2012(7) [2]Zaditen(Ketotifen),as mast cell blocker,improves sperm quality,chromatin integrity and pregnan...
tablishedapharmicmodelonthedegranulationofRBL-2H3mastcells,activatedbycompound48/80(10 mL).Pretreated RBL-2H3cellswithdiferentdilutionsofpaeonol,Thehistamineandtumornecrosisfactor—alpha(TNF— )fromthesupernatants, thespontaneouslyreleasedhistamineandthetotalhistaminewerealldeterminedbyELISA.Results ...
Passante E , Ehrhardt C , Sheridan H & Frankish N ( 2009 ). Toll-like receptors and RBL-2H3 mast cells . Inflamm res 58 Suppl 1 , S11 – S12 .Passante E, Ehrhardt C, Sheridan H and Frankish N. Toll-like receptors and RBL-2H3 mast cells. Inflamm Res 2009; 58(Suppl. 1): 11...
(rat peritoneal mast cells,RPMCs)对 Compound 48/80 响应性的差异.结果 RBL-2H3 细胞可 表达 MrgprB2 受体.Compound 48/80 能剂量依赖性地诱导 RBL-2H3 细胞释 放β-己糖胺酶,但在高剂量(≥20 mg·L-1)时对 RBL-2H3 的细胞活力产生明显影 响,此时释放的 β-己糖胺酶应当是由于其细胞毒作用引起...
mast cell activation induced by IgE cross-linking. The widely used RBL-2H3 mast cells were sensitized with anti-DNP (dinitrophenyl) IgE overnight and activated with DNP-BSA (bovine serum albumin) for up to 4 h. The untreated cells and those with anti-DNP IgE sensitization but without DNP-...
Recent studies have shown that, in mast cells, membrane microdomains rich in cholesterol and glycosphingolipids called lipid rafts play an important role in FcepsilonRI signaling. The present study demonstrates that, in RBL-2H3 cells following stimulation, the mast cell-specific gangliosides associ...
An increase in concentration of cytosolic Ca2+ ([Ca2+]i) is associated with an accelerated influx of 45Ca2+ when cultured RBL-2H3 cells are stimulated with either antigen or analogs of adenosine although these agents act via different receptors and coupling proteins (Ali, H., Cunha-Melo, J...
RBL 2H3 cells (a model of mast cell function) were sensitized with anti-TNP IgE (0.5 micrograms/ml) and triggered to secrete both histamine and arachidonic... PY Lin,GA Wiggan,AM Gilfillan - 《Journal of Immunology》 被引量: 176发表: 1991年 DNP-phycobiliproteins, fluorescent antigens to...
Lyn is necessary for Fc蔚RI-mediated mast cell activation. To investigate how the level of Lyn is maintained in mast cell activation, it was studied whether Lyn binds to ubiquitin and is ubiquitinated for proteasomal degradation in cells. In the yeast two hybrid system, Lyn specifically ...