[3]The RBL-2H3 cell line:its provenance and suitability as a model for the mast cell[J].Egle Passante,Neil Frankish.Inflammation Research.2009(11) [4]RBL-2H3 cells are an imprecise model for mast cell mediator release[J].Egle Passante,Carsten Ehrhardt,Helen Sheridan,Neil Frankish.Inflammatio...
Passante, E.; Ehrhardt, C.; Sheridan, H.; Frankish, N. RBL-2H3 cells are an imprecise model for mast cell mediator release. Inflamm. Res. 2009, 58, 611-618. [CrossRef] [PubMed]Passante E, Ehrhardt C, Sheridan H, et al. RBL-2H3 cells are an imprecise model for mast cell ...
Methods RBL-2H3 mast cells were divided into control group (PBS), C48/80 (positive control, 4 mg/mL) group and salvianolic acid, salvianolic acid B and salvianolic acid D groups at gradient concentrations (0.025, 0.050, 0.100, 0.200, 0.400, 0.800 mg/mL) for injection. After incubation ...
Conclusion: Tween 80 can induce RBL-2H3 cells degranulation directly, and the degree of effect is related to the different quality among these products. Cell degranulation induced by Tween 80 (Shanghai) dues to its cytotoxicity. Key words Tween 80; pseudoallergy; mast cells; β-hexosaminidase;...
摘要：肥大细胞在过敏性疾病发生中起关键作用，而ＲＢＬ－２Ｈ３细胞系作为大鼠嗜碱性粒细胞系（ｒａｔ ｂａｓｏｐｈｉｌｉｃ ｌｅｕｋｅｍｉａ，ＲＢＬ）的一个亚系，可以表达肥大细胞的许多特性和功能，因此常作为过敏性疾病研究的替代模型。ＲＢＬ－２Ｈ３细胞系常用作过敏反应中肥大细胞脱颗粒和激活机制方面的...
结论:一些TCMI能引起体外RPMC和RBL-2H3细胞脱颗粒,2种细胞脱颗粒方法在评价一些TCMI可能引起类过敏反应方面具有一定的互补性与可行性。类过敏反应;中药注射剂;肥大细胞脱颗粒;RBL-2H3细胞;β-氨基己糖苷酶释放率R283.6A1005-9903(2011)22-0152-06StudiesonDegranuationofRatPeritonealMastCellsandRBL-2H3CellsCausedby...
Passante E , Ehrhardt C , Sheridan H & Frankish N ( 2009 ). Toll-like receptors and RBL-2H3 mast cells . Inflamm res 58 Suppl 1 , S11 – S12 .Passante E, Ehrhardt C, Sheridan H and Frankish N. Toll-like receptors and RBL-2H3 mast cells. Inflamm Res 2009; 58(Suppl. 1): 11...
Cytotoxic effect of GSE on RBL-2H3 cells The MTT assay was used to access the cytotoxicity of GSE on RBL-2H3 mast cells. GSE in the range of 1–100 μg/ml did not cause significant cytotoxicity after 24-h treatment as compared with the control (Fig. 1), whereas GSE used at higher...
RBL-2H3 cells are an imprecise model for mast cell mediator release. Inflamm. Res. 2009, 58, 611-618. [CrossRef] [PubMed]Egle Passante,Carsten Ehrhardt,Helen Sheridan,Neil Frankish.RBL-2H3 cells are an imprecise model for mast ... E Passante,C Ehrhardt,H Sheridan,... - Inflammation ...
用显微镜观察和 MTS 法考察 Compound 48/80 对 RBL-2H3 细胞活力的影响;测定不同浓度 Compound 48/80 刺激 RBL-2H3 细胞脱颗粒释放的 β-己糖胺酶含量,对比 RBL-2H3 细胞、人肥大细胞系 LAD2 和大鼠腹腔肥大细胞(rat peritoneal mast cells,RPMCs)对 Compound 48/80 响应性的差异.结果 RBL-2H3 细胞可...