These 铿乶dings imply that isoleucine downregulates the expression of gluconeogenic rate-limiting enzyme genes and inhibits glucose production in the liver. We also compared the gene expression profiles in the liver of isoleucine-administered rats and fasted rats by DNA microarray. The gene expression ...
A method is proposed to detect whether a given enzyme catalyzes a rate-limiting step in a metabolic pathway. With the use of a range of concentrations of specific inhibitors of an enzyme, the finding of a biphasic response with an initial null effect indicates the non-rate-limiting nature of...
Fructose 1,6-diphosphatase has been isolated from swine kidney extracts by a procedure which yields large quantities of homogeneous enzyme. The purified preparation has an extinction coefficient () of 8.9 in 0.05 Tris-HCl, pH 8.0 and a specific activity of 25 to 30 µmoles per min per mg...
What is a type of enzyme regulation in which the accumulation of the product of a reaction inhibits an earlier reaction in the sequence? A chemical reaction between two atoms occurs because A. the two atoms forcibly collide with each other. B. th...
The Production of a Rat Model That Inhibits Phosphoenolpyruvate Carboxykinase (PEPCK), a Rate-Limiting Enzyme of Hepatic GluconeogenesisHepatic glucose productionProlonged exerciseRunning performanceWe previously showed that taurine administration contributed to the extension of time to exhaustion through exercise...
The Production of a Rat Model That Inhibits Phosphoenolpyruvate Carboxykinase (PEPCK), a Rate-Limiting Enzyme of Hepatic GluconeogenesisHepatic glucose productionProlonged exerciseRunning performanceWe previously showed that taurine administration contributed to the extension of time to exhaustion through exercise...
GluconeogenesisDose-Response Relationship, DrugA method is proposed to detect whether a given enzyme catalyzes a rate-limiting step in a metabolic pathway. With the use of a range of concentrations of specific inhibitors of an enzyme, the finding of a biphasic response with an initial ...
Because the glucose-transport-hexokinase segment is not rate limiting, phosphofructo 1-kinase has almost as much control strength as it would have as the first enzyme of the pathway, and because the F6P/FDP cycle is only moderately rate limiting, Fru-1,6-P2ase and enzymes further downstream ...