英文名Rat GAPDH Endogenous Reference Genes Primers, 100 μM 相关类别内参引物储存冷冻(-20℃) 编 号包装库存目录价(¥)您的价格(¥)数量 B662204-0001100 ul现货600600 产品描述 概述 实时荧光定量PCR (定量PCR)技术能够非常灵敏的对核酸进行定量。在研究不同细胞类型,发育阶段,和/或样品处理时,内源性参照基...
The method comprises the steps of: separately designed rats each selenoprotein gene and the reference gene Actb, Gapdh an mRNA qPCR primer, in addition to non-intron genes, at least one of cross-Miscellaneous the qPCR primers for each gene in the qPCR primer containing the gene; the ...
Total RNA isolation, cDNA synthesis and qPCR were performed as previously described44,45. The primers for qPCR are listed as follows: rat GAPDH (5′-3′) F: GGCACAGTCAAGGCTGAGAATG, R: ATGGTGGTGAAGACGCCAGTA; rat SHBG (ABP) (5′-3′) F: ATGTGGACCTGCAACCTGGAC, R: TGCTCCATCCACCAGCT...
The GAPDH gene was used as an internal standard gene. The results were acquired after 40 cycles of amplification. The threshold cycles (Ct) were conclusive for each gene and gene expression levels were evaluated using 2–ΔΔCt. Quantification data represented the mean of triplicated experiments....
Primers targeting rat GAPDH were also prepared: forward primer, 5′-GAACATCATCCCTGCATCCA-3′; reverse primer, 5′-CCAGTGAGCTTCCCGTTCA-3′. PCR amplifications were performed using the following program: initial denaturation (10 min, 95°C), followed by 40 cycles (15 s, 95°C; 1 min, ...
Expression levels were calculated relative to the expression of the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with Bio-Rad CFX-Manager (Bio-Rad, USA). The relative expression levels of genes were calculated using the 2-∆∆Ct method. The primer sequences used in these...
RNA integrity was evaluated by qPCR comparison of the relative levels of the 3′ and 5′ ends of the transcripts for the housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (primers are listed in Table 3), as previously described [82]. RNA samples were further ...
GAPDH was used for qPCR normalization. Table 1 The sequences of the primers. Full size table Western blotting The western blot analysis was conducted according to Taylor et al.’s method20 with a GAPDH monoclonal antibody (1:5,000, Abcam, ab181602) used as an internal control. The ...
cDNA transcripts were quantified using real-time quantitative PCR with Power SybrGreen® Master Mix (Applied Biosystems) using gene specific primers (Supplementary Table 1) and normalized to glyceraldehyde-3-phosphate dehydrogenase (Gapdh) mRNA. miRNA isolation was performed for in vitro and in vivo...
Table 3 Primer sequences used for qPCR amplification. Full size table Microarray analysis To compare the gene expression of cells in each group, microarray analysis was performed by Shanghai OE Biotech Co., Ltd. Simply, the total RNA was extracted according to the method mentioned above and its...