碧云天生产的Active Ras Pull-down and Detection Kit,也称活性Ras沉淀与检测试剂盒,是一种便捷、高效的用于定量检测组织或细胞裂解液中激活状态(Active state) Ras蛋白(包括NRas、HRas和KRas)的试剂盒。本试剂盒基于Raf1-RBD Agarose (活性Ras结合琼脂糖凝胶)特异性地结合沉淀(Pull-down)激活状态的Ras蛋白,通过Weste...
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The Thermo Scientific Active Ras Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Ras GTPase through a specific protein interaction with the Raf1 protein-binding domain.The Active Ras Pull-Down and Detecti
16117 ACTIVE RAS PULL-DOWN AND DETECTION KIT 8928 DETECTION KIT Pierce 16116 ACTIVE RHO PULL-DOWN AND DETECTION KIT 8952 DETECTION KIT Pierce 16119 ACTIVE CDC42 PULL-DOWN AND DETECTION KIT 8952 DETECTION KIT Pierce 16120 ACTIVE RAP1 PULL-DOWN AND DETECTION KIT 8952 DETECTION KIT Pierce 16121 AC...
具体操作步骤,请参考阿拉丁Active Ras Pull-down and Detection Kit 说明书。参考文献:1.Tran TH, Chan AH, Young LC, Bindu L, Neale C. Nat Commun. 2021. 12(1):1176.2.Vernoud V, Horton AC, Yang Z, Nielsen E. Plant Physiol. 2003. 131(3):1191-208.3.Ito H, Morishita R, Nagata KI. ...
Active Ras Pull-down and Detection Kit enables selective enrichment and detection of Ras GTPase. Sufficient for 50 pull-down assays.
The abundance of the active GTP-bound form of RAS was analyzed using an Active RAS Pull-Down and Detection Kit (Thermo Fisher Scientific, Cat# 16117) according to the manufacturer’s instructions. Cell lysates containing 1.0 mg of protein were incubated with 50 μl of Raf1-RBD-agarose...
The pull-down assay was conducted using an Active Ras Pull-Down and Detection kit (16117, Thermo Fisher Scientific) according to the manufacturer’s instructions, and proteins were recovered from the resultant immunoprecipitates in 2× SDS sample buffer. Immunoblotting Samples were subjected to SDS...
The present invention belongs to the field of molecular biology, and discloses a method and kit for detecting Ras activity of a small molecular G protein by utilizing GST pull-down and Western-Blot. The kit comprises: a conventional GST pull-down experimental components, a GST-Raf1-RBD ...
8D). Importantly, active Ras pull-down assays confirmed a role for RasGRP3 in Ras activation, since knockdown of RasGRP3 reduced the level of activated Ras seen in PMA-treated cells (Fig. 8D). RasGRP3 is partially responsible for PKCα-induced antiproliferative signaling in intestinal ...