碧云天生产的Active Ras Pull-down and Detection Kit,也称活性Ras沉淀与检测试剂盒,是一种便捷、高效的用于定量检测组织或细胞裂解液中激活状态(Active state) Ras蛋白(包括NRas、HRas和KRas)的试剂盒。本试剂盒基于Raf1-RBD Agarose (活性Ras结合琼脂糖凝胶)特异性地结合沉淀(Pull-down)激活状态的Ras蛋白,通过Weste...
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and that its binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of Ras make it an ideal tool for affinity purification of GTP-Ras from cell lysates. The Raf-RBD is in the form of a GST fusion protein, which allows one to "pull-down" the Raf-RBD/GTP-...
16117 ACTIVE RAS PULL-DOWN AND DETECTION KIT 8928 DETECTION KIT Pierce 16116 ACTIVE RHO PULL-DOWN AND DETECTION KIT 8952 DETECTION KIT Pierce 16119 ACTIVE CDC42 PULL-DOWN AND DETECTION KIT 8952 DETECTION KIT Pierce 16120 ACTIVE RAP1 PULL-DOWN AND DETECTION KIT 8952 DETECTION KIT Pierce 16121 AC...
The Thermo Scientific Active Ras Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Ras GTPase through a specific protein interaction with the Raf1 protein-binding domain.The Active Ras Pull-Down and Detection Kit includes purified GST-Raf1 Ras-binding domain (RBD)...
为了研究Ras的活性,研究者们普遍用Ras结合域(RBD)来进行pulldown实验,但是这种方法的时空分辨率很差,而且无法在细胞环境中研究靶点的活性,所以需要开发新方法。目前,基因编码的荧光生物传感器已成为研究生命系统中信号分子时空调控的有力工具,但...
(H)如通过CaM pull-down分析所揭示的,通过prostratin处理抑制钙调蛋白与K-RasV12的相互作用。WCB:全细胞裂解物。IB:免疫印迹。(I)在K-RasV12而非K-RasV12-S181A突变体或H-RasV12转化的NIH/3T3细胞中,通过prostratin处理升高的CaMKii活性。(J)在对prostratin处理的应答中,K-RasV12、K-RasV12-S181A突变...
2.1蛋白质体外GST-pull down实验 利用谷胱甘肽S-转移酶(GST)基因融合载体体外表达纯化出GST、和GST-Nedd4-1 WT、GST-Nedd4-1 CA融合蛋白,同时体外纯化GST-Flag-Ras蛋白,根据蛋白质体外GST pull-down反应体系,在TNTE 0.5%反应缓冲液中,加入相应量的纯化蛋白。旋转摇床4℃孵育1～2小时,4℃,2400rpm离心1分钟,弃...
PulldownassayRho,Raf(Ras),PAK(Rac)Growcellstonearconfluencyon10cmdishesfor2-4daysReplacegrowthmediawithserumfreemedia3-5hrsbeforestartingexperiment..