we formally tested for gene flow within theSyzygium grandegroup using Patterson’sf3statistic56, which tests for patterns of allele sharing (source data are provided at Dryad,https://doi.org/10.5061/dryad.h18931zpw). We calculated all three-way taxon comparisons of source1, source2, and target...
DNA-based methods such as the polymerase chain reaction (PCR) have increasingly been used for rapid and sensitive detection of L. monocytogenes. Among the various available PCR assays, we used the BAX ® system (with two different detection procedures: gel detection and automated detection) to ...
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Two different reference standard RT-PCR tests were used to evaluate the performance of Saliva-Dry LAMP. In the first reference RT-PCR, RNA extracted from saliva samples using the streamlined column-based extraction which was used for Saliva-Dry LAMP was also used in RT-PCR reactions. This asse...
The study also determined, Siddiqui says, that the negative predictive value (NPV—the probability that a person who tests negative has not been infected with SARS-CoV-2, as PCR would show) was high for both symptomatic (99.2%) and asymptomatic (99.7) young people. ...
sheen Ps float Amount of soft reflection near edges clearcoat_thickness Pc float Extra white specular layer on top clearcoat_roughness Pcr float Roughness of white specular layer anisotropy aniso float Anisotropy for specular reflection anisotropy_rotation anisor float Anisotropy rotation amount roughness...
We undertook a nested case-control study derived from a cohort of 400 healthcare workers at one London hospital recruited from March 23, 2020 to undergo weekly nasopharyngeal swab PCR tests and blood sampling when fit to attend work, as described previously.6, 7, 8, 9, 10 In this cohort,...
Although the results of both quantitative and qualitative tests can be obtained within 15 min, the principal disadvantages of a quantitative test are the higher cost and requirement for a specific instrument [30]. The present study was conducted with the aim to evaluate two rapid diagnostic test ...
The MinION sequencing platform offers near real-time analysis of DNA sequence; this makes the tool attractive for deployment in fieldwork or clinical settings. We used the MinION platform coupled to the NanoOK RT software package to perform shotgun metag
While molecular surveillance methods such as rapid PCR tests and real-time genome sequencing can identify the presence of known resistance genes28,29,30,31, e.g., efflux pumps, to identify resistant strains, they are not suitable for monitoring within-population pathogen diversity. Furthermore, ...