Rapid PCR detection of the methicillin resistance gene, mecA, on the hands of medical and non- medical personnel and healthy children and on surfaces in a neonatal intensive care unit. Scand J Infect Dis. 2001;33(7):494-7.Klingenberg C, Glad GT, Olsvik R. Flaegstad T. Rapid PCR ...
On the right, qRT-PCR measurements of LOLI1 with indicated sgRNAs; n = 3 (experimental replicates). i The effect of CRISPRa on HeLa cells’ viability; n = 6 (experimental replicates). Statistical significance was estimated by one-sided paired t test at 48 h. j Plasmids ...
Genetic code expansion (GCE) offers many exciting opportunities for the creation of synthetic organisms and for drug discovery methods that utilize in vitro translation. One type of GCE, sense codon reassignment (SCR), focuses on breaking the degeneracy
The >1000 genes detected threshold was selected due to rapid decay of complexity below 1000 genes, potentially due to failed lysis of some cells. B Distribution of numbers of ERCC species detected in each of the 276 neocortex single cells analyzed. The set of cells at 0 ERCCs represents ...
"oncogene addiction", that many cancers are dependent upon a small set of key genes to drive their rapid cellular multiplication with the rest of the mutations simply being passenger mutations [2,3], the identification of driver oncogenic mutations has become of critical importance in cancer ...
RT-PCR has been successfully utilised to identify positive cases with a sensitivity of 100 % and a specificity of 85–100 %, using FISH as the reference standard method [37, 42]. Multiplex RT-PCR is easy to perform, rapid and relatively inexpensive but may be challenging using RNA ...
The rapid advancements in methodologies such as MeDIP and real time qPCR, next generation sequencing, digital PCR, array-CGH and other emerging technologies will permit within the next 10 years, provision of NIPD for all other common and rare genetic disorders. Therefore, the continuous development...
The gel content was transferred to a nitrocellulose membrane using the rapid transfer method. Nitrocellulose membranes were incubated in blocking buffer (5% BSA, 0.1% tween in PBS) for 1 hour, at room temperature on a tilt table. The membranes were then exposed to the appropriate primary ...
Three-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Live cell imaging has become the leading method for chromatin dynamics tracking. However, existing CRISPR- and TALE-based genomic labeling techniques h
on immune checkpoints and their inhibitors has entered a period of rapid development. After a decade of development, several new immune checkpoints have been identified, and monoclonal antibody drugs or small molecule inhibitors targeting them have also been developed. Among them, the immune ...