Species radiations—wherein perplexing amounts of diversity appear to have formed extremely rapidly—have featured prominently in the history of evolutionary theory1. Various underlying mechanisms for their formation have been proposed2, including adaptation2, non-adaptive processes3,4, hybridisation5,6,...
Rapid and sensitive identifi- cation of buffalo's, cattle's and sheep's milk using species-spe- cific PCR and PCR-RFLP techniques. Food Control. 2007;18: 1246-9.SM Abdel-Rahman,MM Ahmed.Rapid and sensitive identification of buffalo’s, cattle’s and sheep’s milk using species-specific...
the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought to develop a rapid...
Testing was then carried out to determine whether this immuno- Comparison of the detection sensitivity In an effort to address the shortcoming of TCVA, which is the long duration of testing, substantial research has been conducted to test the application of PCR or real- time PCR methods as ...
We targeted three fibroin genes for silencing and measured their expression levels by qRT-PCR 4 days after injection (Fig. 5b). In comparison with the noninjected groups, there were no significant changes in the expression of GFP, HcunFib-L and HcunP25 (p > 0.05), while the relative...
For the rapid, specific and sensitive identification of buffalo's, cattle's and sheep's milk, species-specific PCR and PCR-RFLP techniques were developed. DNA from small amount of fresh milk (100 micro L) was extracted to amplify the gene encoding species-specific repeat (SSR) region and ...
For each lot of lentiviral vector, titer was detected by measuring the level of HIV-1 Gag p24 protein using the RETROtek HIV-1 p24 Antigen-ELISA kit (Zeptometrix; Buffalo, NY), according to manufacturer’s instructions. Indirect ImmunoFluorescence Test (IIFT) To determine the reactivity and ...
Genotypes of a subset of these mice, and of littermates for breeding and control experiments, were determined by PCR using the primers listed in Supplementary Table 1. Perfusion and immunohistochemistry. Naive or previously imaged or recorded mice were deeply anaesthetized with 200 mg kg À 1 ...
CaMKIIα-sypGFP-tdTom and Gγ8-ChIEF-Citrine pups were detected by visualization of fluorescence in the nose and OB of P0-2 pups under epifluorescence illumination. Genotypes of a subset of these mice, and of littermates for breeding and control experiments, were determined by PCR using the...
2.5. Limit of Detection (LOD) of GenBody™ COVID-19 Ag Test To determine the LOD of the developed kit, cultured and inactivated SARS-CoV-2 virus obtained from Zeptomatrix Inc. (Buffalo, NY, USA) and the recombinant SARS-CoV-2 NP from the previous study [12], along with anti-NP MA...