We generated PCR primers to test for the presence or absence of each insertion, and amplified fragments from genomic DNA using both strains. In all cases the PCR tests verified the presence of the insertion in one strain and its absence in the other (data not shown). Regions of lateral ...
Kaplan:When the season began, all players and coaches were administered PCR tests daily. While PCR tests are quite accurate, there is a 12- to 24-hour turnaround for results, and many in the NHL became concerned about that lag time. It seemed plausible that infected...
theSbf1restriction site were also discarded. Reads were filtered to remove PCR duplicates using the STACKSclone_filterscript. The remaining reads were then mapped to theZosterops lateralis melanopsgenome assembly version 1 (NCBI Assembly GCA_001281735.1) (Cornetti et al.2015) with BOWTIE2 version ...
(Applied Biosystems, Woburn, MA, USA), following the manufacturer’s protocol. Extracted nucleic acids were transferred to LoBind tubes, quantified using Qubit 1X dsDNA high sensitivity kit (Thermo Fisher Scientific, Waltham, MA, USA), and used for PCR, to generate B646L (p72) amplicons, ...
Applied Predictive Modeling, 2nd ed.; Springer: Manhattan, NY, USA, 2016. 32. Lee, J.E.; Kim, K.S.; Shin, H.W.; Sohn, Y.H. Factors Related to Clinically Probable REM Sleep Behavior Disorder in Parkinson Disease. Parkinsonism. Relat. Disord. 2010, 16, 105–108. [CrossRef] 33. ...